Enhancing Synovial Mesenchymal Stem Cell Adhesion and Selection via an Avidin-Biotin-CD105 Binding System for Cartilage Tissue Engineering

被引：8
作者：
Fan, Wenshuai [1 ]
Wang, Yiming [1 ]
Yuan, Liu [2 ,3 ]
Li, Shuo [1 ]
Pan, Jianfeng [1 ]
Chen, Chen [1 ]
Yan, Zuoqin [1 ]
Guo, Changan [1 ]
机构：
[1] Fudan Univ, Zhongshan Hosp, Dept Orthoped Surg, Shanghai 200032, Peoples R China
[2] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Biomat & Tissue Engn Lab, Shanghai 201620, Peoples R China
[3] Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China
来源：
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2016年 / 6卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Cartilage Repair; Mesenchymal Stem Cells; Avidin-Biotin; CD105; Antibody; Cell Adhesion; SCAFFOLDS;
D O I：
10.1166/jbt.2016.1401
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Efficient attachment of cells to a supporting scaffold is the critical step in many cartilage tissue engineering strategies. The present study aimed to investigate the effects of a novel avidin-biotin-conjugated CD105 antibody binding system (CD105-ABBS) on synovial mesenchymal stem cell (SMSC) adhesion and selection in cartilage tissue engineering. SMSC adhesion on cell culture plates or porous scaffolds was assessed by counting attached cells, and CD105-positive cells on cell culture plates as well as sulfated glycosaminoglycan (sGAG) secretion in porous scaffolds were evaluated by immunofluorescence and the 1, 9-dimethylmethylene blue binding assay. Finally, defects created in the femoral condyle of beagles were repaired using cell-laden scaffolds employing different binding system, and the contents of sGAG and collagen II in the engineered cartilage were evaluated. The results indicated that the CD105-ABBS improved SMSC adhesion, especially at early time points after seeding. In addition, the CD105-ABBS promoted the selection of CD105-positive cells as well as the secretion of sGAG. Moreover, the reparative effects of implanted SMSCs were improved with the use of the CD105-ABBS in vivo. Therefore, the results of this study suggest that this new binding system can not only enhance SMSC adhesion, but also improve selection of a specific subset of SMSCs, in support of cartilage repair.
引用
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页码：27 / 34
页数：8
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