Biosensor for label-free DNA quantification based on functionalized LPGs

被引：43

作者：

Goncalves, Helena M. R. ^{[1
]}

Moreira, Luis ^{[1
]}

Pereira, Leonor ^{[1
,3
]}

Jorge, Pedro ^{[2
]}

Gouveia, Carlos ^{[2
]}

Martins-Lopes, Paula ^{[1
,3
]}

Fernandes, Jose R. A. ^{[1
,2
]}

机构：

[1] Univ Tras Os Montes & Alto Douro, POB 1013, P-5000911 Vila Real, Portugal

[2] INESC TEC, Rua Campo Alegre 687, P-4169007 Oporto, Portugal

[3] Univ Lisbon, Fac Sci, BioISI Biosyst & Integrat Sci Inst, P-1699 Lisbon, Portugal

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 84卷

关键词：

Biosensing; Label-free; Detection; Quantification; DNA; DIRECT ELECTROCHEMICAL SENSOR; GRATING SENSORS; FIBER; REAGENTLESS; RECOGNITION; GROWTH; ARC;

D O I：

10.1016/j.bios.2015.10.001

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：30 / 36

页数：7

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