Inhibitory Activity of Brown Propolis Extracts on a Norfloxacin-Resistant Strain of Staphylococcus aureus

被引：4

作者：

Frota, Vanessa Moreira ^{[1
]}

Dias, Francisco Matheus F. ^{[1
]}

do Nascimento, Mariana Ferreira ^{[1
]}

da Silva Mendonca, Lavosyer ^{[1
]}

dos Santos Moita, Emanuella Cristina ^{[1
]}

dos Santos Gomes, Laressa Cristyne ^{[2
]}

Leal, Antonio Linkoln Alves Borges ^{[3
]}

Barreto, Humberto Medeiros ^{[3
]}

Silva, Maria Francilene Souza ^{[4
]}

dos Santos Fontenelle, Raquel Oliveira ^{[2
]}

Gomes, Geovany Amorim ^{[1
]}

do Vale, Jean Parcelli C. ^{[1
]}

da Silva Juliao, Murilo Sergio ^{[1
]}

Bandeira, Paulo Nogueira ^{[1
]}

Pessoa, Claudia do O. ^{[4
]}

dos Santos, Helcio Silva ^{[1
]}

Zocolo, Guilherme Juliao ^{[5
]}

Rodrigues, Tigressa Helena S. ^{[1
]}

机构：

[1] Univ Estadual Vale Acarau, Curso Quim, Ctr Ciencias Exatas & Tecnol, Ave Univ 850, BR-62 04037 Sobral, Ceara, Brazil

[2] Univ Estadual Vale Acarau, Curso Ciencias Biol, Ctr Ciencias Agr & Biol, Ave Univ 850, BR-62 04037 Sobral, Ceara, Brazil

[3] Univ Fed Piaui, Dept Parasitol & Microbiol, Campus Minist Petronio Portella, BR-64049550 Teresina, Piaui, Brazil

[4] Univ Fed Ceara, Fac Med, Dept Fisiol & Farmacol, Rua Coronel Nunes Melo 1127, BR-60 43027 Fortaleza, Ceara, Brazil

[5] Embrapa Agroind Trop, Rua Doutora Sara Mesquita 2270, BR-60 02018 Fortaleza, Ceara, Brazil

来源：

REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2021年 / 31卷 / 02期

关键词：

Phenolics; Volatiles; Non-volatile compounds; Efflux pump inhibitors; Cytotoxicity;

D O I：

10.1007/s43450-021-00150-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Brown propolis from northern Ceara, Brazil, was found to contain predominantly sesquiterpenes in its volatile profile. The ethanolic propolis extract yielded a substantial apolar fraction (80.07 +/- 1.47%) after liquid-liquid partitioning, which explains the low phenolic content (41.33 +/- 1.68 mg(GAE) g(EXT)(-1)). Phenolics, such as p-coumaric (0.13 +/- 0.04 mg g(EPE)(-1)), ferulic (1.07 +/- 0.09 mg g(EPE)(-1)), and trans-cinnamic (0.41 +/- 0.02 mg g(EPE)(-1)) acids, were detected in the EtOH-soluble extract. The apolar fraction was rich in triterpenes such as cycloartenol (12.85 +/- 2.80%), alpha-amyrenyl acetate (4.37 +/- 0.69%), and alpha-amyrin (3.89 +/- 0.62%). Despite the lack of a direct antibacterial activity against Staphylococcus aureus 1199-B, both fractions exhibited potentiating effects in combination with norfloxacin; these effects could be attributed to efflux pump inhibition. The apolar fraction was more effective than the EtOH-soluble one at reducing the minimum inhibitory concentration, producing similar results to chlorpromazine and ethidium bromide. The EtOH-soluble fraction exhibited cytostatic properties at lower concentrations, while the apolar fraction demonstrated better selectivity for PC3 and HL60 tumor cells. The findings highlight the biological potential of Brazilian brown propolis for application as a complementary agent in the treatment of S. aureus infections and as an alternative therapy for cancer treatment.

引用

页码：249 / 255

页数：7

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