Temperature acclimation and adaptation of enzyme physiology in Neurospora discreta

被引：11

作者：

Allison, Steven D. ^{[1
,2
]}

Romero-Olivares, Adriana L. ^{[1
]}

Lu, Lucy ^{[1
]}

Taylor, John W. ^{[3
]}

Treseder, Kathleen K. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92697 USA

[3] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

来源：

FUNGAL ECOLOGY | 2018年 / 35卷

关键词：

Climate change; Experimental evolution; Extracellular enzyme; Km; Neurospora discreta; Physiological acclimation; Respiration; Soil carbon; Thermal adaptation; Vmax; CLIMATE-CHANGE; BOREAL FOREST; CATALYTIC EFFICIENCY; FUNGAL COMMUNITY; GENE-EXPRESSION; DECOMPOSITION; ECOSYSTEM; DYNAMICS; LITTER; CRASSA;

D O I：

10.1016/j.funeco.2018.07.005

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Fungal metabolic rates could increase under climate warming but may be counteracted by mechanisms of physiological acclimation and evolutionary adaptation. We hypothesized that Vmax and Km parameters of Neurospora discreta extracellular enzymes would acclimate to warmer temperatures through compensatory mechanisms. We also predicted that evolution under warmer temperatures would alter enzyme parameters and fungal respiration through adaptive mechanisms. In contrast to these predictions, growth at higher temperature (22 degrees C versus 16 degrees C) increased the temperature-corrected Vmax of three enzymes. The carbon substrate used for fungal growth (lignin versus sucrose) had a much greater impact on enzyme Vmax than temperature. Following experimental evolution, the enzymatic parameters of Neurospora strains did not adapt to higher temperatures as hypothesized; rather, enzyme Vmax values were unaffected, and respiration rates increased. Together, these results suggest that physiological and evolutionary mechanisms are unlikely to counteract soil carbon losses driven by saprotrophic fungi under climate warming. (C) 2018 Elsevier Ltd and British Mycological Society. All rights reserved.

引用

页码：78 / 86

页数：9

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