Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion

被引:40
作者
Atshaves, BP
Foxworth, WB
Frolov, A
Roths, JB
Kier, AB
Oetama, BK
Piedrahita, JA
Schroeder, F [1 ]
机构
[1] Texas A&M Univ, Texas Vet Med Ctr, Dept Physiol & Pharmacol, College Stn, TX 77843 USA
[2] Texas A&M Univ, Texas Vet Med Ctr, Dept Pathobiol, College Stn, TX 77843 USA
[3] Texas A&M Univ, Texas Vet Med Ctr, Dept Anat & Publ Hlth, College Stn, TX 77843 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1998年 / 274卷 / 03期
关键词
embryonic stem cells; intracellular localization; laser cytometry; fluorescence recovery after photobleaching; intestinal fatty acid binding protein;
D O I
10.1152/ajpcell.1998.274.3.C633
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effect of cellular differentiation on fatty acid uptake and intracellular diffusion was examined in transfected pluripotent mouse embryonic stem (ES) cells stably expressing intestinal fatty acid binding protein (I-FABP). Control ES cells, whether differentiated or undifferentiated, did not express I-FABP. The initial rate and maximal uptake of the fluorescent fatty acid, 12-(N-methyl)-N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-octadecanoic acid (NBD-stearic acid), was measured in single cells by kinetic digital fluorescence imaging. I-FABP expression in undifferentiated ES cells increased the initial rate and maximal uptake of NBD-stearic acid 1.7- and 1.6-fold, respectively, as well as increased its effective intracellular diffusion constant (D-eff) 1.8-fold as measured by the fluorescence recovery after photobleaching technique. In contrast, ES cell differentiation decreased I-FABP expression up to 3-fold and decreased the NBD-stearic acid initial rate of uptake, maximal uptake, and D-eff by 10-, 4.7-, and 2-fold, respectively. There were no significant differences in these parameters between the differentiated control and differentiated I-FABP-expressing ES cell lines. In summary, differentiation and expression of I-FABP oppositely modulated NBD-stearic acid uptake parameters and intracellular diffusion in ES cells.
引用
收藏
页码:C633 / C644
页数:12
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