2-C-Methyluridine modified hammerhead ribozyme against the estrogen receptor

被引：8

作者：

Pontiggia, Rodrigo ^{[1
]}

Pontiggia, Osvaldo ^{[2
]}

Simian, Marina ^{[2
]}

Montserrat, Javier M. ^{[1
,3
]}

Engels, Joachim W. ^{[4
]}

Iribarren, Adolfo M. ^{[1
,5
]}

机构：

[1] Consejo Nacl Invest Cient & Tecn, INGEBI, RA-1428 Buenos Aires, DF, Argentina

[2] UBA, Inst Oncol AH Roffo, Caba, Argentina

[3] Univ Nacl Gral Sarmiento, Inst Ciencias, Prov De Bs As, Argentina

[4] Goethe Univ Frankfurt, Inst Organ Chem & Chem Biol, D-60438 Frankfurt, Germany

[5] Univ Nacl Quilmes, Lab Biotransformac, RA-1876 Bemal, Prov De Bs As, Argentina

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2010年 / 20卷 / 09期

关键词：

2 '-C-methyluridine; Modified ribozyme; Estrogen receptor alpha; RNA; 2'-C-METHYLURIDINE; GLUCOSE;

D O I：

10.1016/j.bmcl.2010.03.060

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A new synthesis of 2'-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5'-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor alpha protein (ER-alpha), and transfection experiments in MCF-7 cell line are also presented. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2806 / 2808

页数：3

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