Kinetics of the early events of GPCR signalling

被引：12

作者：

Adamson, Roslin J. ^{[1
]}

Watts, Anthony ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, Biomembrane Struct Unit, Oxford OX1 3QU, England

来源：

FEBS LETTERS | 2014年 / 588卷 / 24期

基金：

英国惠康基金;

关键词：

G protein-coupled receptor; Electron microscopy; Surface plasmon resonance; Nanodisc; G protein; PROTEIN-COUPLED RECEPTORS; DELTA-OPIOID RECEPTOR; MEMBRANE-PROTEINS; PLASMON RESONANCE; LIGAND-BINDING; NEUROTENSIN; NANODISCS; RECONSTITUTION; PURIFICATION; RHODOPSIN;

D O I：

10.1016/j.febslet.2014.10.043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neurotensin receptor type 1 (NTS1) is a G protein-coupled receptor (GPCR) that affects cellular responses by initiating a cascade of interactions through G proteins. The kinetic details for these interactions are not well-known. Here, NTS1-nanodisc-G alpha(s) and G alpha(i1) interactions were studied. The binding affinities of G alpha(i1) and G alpha(s) to NTS1 were directly measured by surface plasmon resonance (SPR) and determined to be 15 +/- 6 nM and 31 +/- 18 nM, respectively. This SPR configuration permits the kinetics of early events in signalling pathways to be explored and can be used to initiate descriptions of the GPCR interactome. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：4701 / 4707

页数：7

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