Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures

被引：19

作者：

Chai, Hua ^{[1
,2
]}

Miao, Peng ^{[1
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China

[2] Jinan Guokeyigong Sci & Technol Dev Co Ltd, Jinan 250103, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2021年 / 32卷 / 02期

关键词：

Circulating tumor DNA; Biosensor; DNA triangular prism; DNA three-way junction; Strand displacement polymerization;

D O I：

10.1016/j.cclet.2020.06.030

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Circulating tumor DNA (ctDNA) refers to a class of acellular nucleic acids carrying genetic features of primary tumor, which can be regarded as a promising noninvasive biomarker for cancer diagnosis. The development of ctDNA assay is an important component of liquid biopsy. In this study, we have fabricated a novel electrochemical strategy for ultrasensitive detection of ctDNA combining the merits of strand displacement amplification and DNA nanostructures. Stable DNA triangular prism is firstly self-assembled and modified on the electrode surface. After target initiated strand displacement polymerization reaction, the generated DNA product helps the formation of three-way junction nanostructure on triangular prism, which localizes electrochemical species. By carefully investigating the electrochemical responses, the limit of detection (LOD) for ctDNA assay as low as 48 amol/L is achieved. This proposed electrochemical biosensor shows great potential for clinical applications. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

引用

页码：783 / 786

页数：4

共 26 条

[1] Integrating genomic features for non-invasive early lung cancer detection [J].

Chabon, Jacob J. ;

Hamilton, Emily G. ;

Kurtz, David M. ;

Esfahani, Mohammad S. ;

Moding, Everett J. ;

Stehr, Henning ;

Schroers-Martin, Joseph ;

Nabet, Barzin Y. ;

Chen, Binbin ;

Chaudhuri, Aadel A. ;

Liu, Chih Long ;

Hui, Angela B. ;

Jin, Michael C. ;

Azad, Tej D. ;

Almanza, Diego ;

Jeon, Young-Jun ;

Nesselbush, Monica C. ;

Keh, Lyron Co Ting ;

Bonilla, Rene F. ;

Yoo, Christopher H. ;

Ko, Ryan B. ;

Chen, Emily L. ;

Merriott, David J. ;

Massion, Pierre P. ;

Mansfield, Aaron S. ;

Jen, Jin ;

Ren, Hong Z. ;

Lin, Steven H. ;

Costantino, Christina L. ;

Burr, Risa ;

Tibshirani, Robert ;

Gambhir, Sanjiv S. ;

Berry, Gerald J. ;

Jensen, Kristin C. ;

West, Robert B. ;

Neal, Joel W. ;

Wakelee, Heather A. ;

Loo, Billy W., Jr. ;

Kunder, Christian A. ;

Leung, Ann N. ;

Lui, Natalie S. ;

Berry, Mark F. ;

Shrager, Joseph B. ;

Nair, Viswam S. ;

Haber, Daniel A. ;

Sequist, Lecia V. ;

Alizadeh, Ash A. ;

Diehn, Maximilian .

NATURE, 2020, 580 (7802) :245-+

[2] Ultrasensitive Detection of ctDNA by Target-Mediated In Situ Growth of DNA Three-Way Junction on the Electrode [J].

Chen, Xifeng ;

Tang, Yuguo ;

Yan, Ruhong ;

Miao, Peng .

CHEMELECTROCHEM, 2020, 7 (01) :64-68

[3] Highly sensitive electrochemical detection of circulating tumor DNA based on thin-layer MoS2/graphene composites [J].

Chu, Yilan ;

Cai, Bin ;

Ma, Ye ;

Zhao, Minggang ;

Ye, Zhizhen ;

Huang, Jingyun .

RSC ADVANCES, 2016, 6 (27) :22673-22678

[4] Dynamic Behavior of DNA Cages Anchored on Spherically Supported Lipid Bilayers [J].

Conway, J. W. ;

Madwar, C. ;

Edwardson, T. G. ;

McLaughlin, C. K. ;

Fahkoury, J. ;

Lennox, R. B. ;

Sleiman, H. F. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (37) :12987-12997

[5] Mapping the breast cancer metastatic cascade onto ctDNA using genetic and epigenetic clonal tracking [J].

Cresswell, George D. ;

Nichol, Daniel ;

Spiteri, Inmaculada ;

Tari, Haider ;

Zapata, Luis ;

Heide, Timon ;

Maley, Carlo C. ;

Magnani, Luca ;

Schiavon, Gaia ;

Ashworth, Alan ;

Barry, Peter ;

Sottoriva, Andrea .

NATURE COMMUNICATIONS, 2020, 11 (01)

[6] Discrimination of the V600E Mutation in BRAF by Rolling Circle Amplification and Forster Resonance Energy Transfer [J].

Dekaliuk, Mariia ;

Qiu, Xue ;

Troalen, Frederic ;

Busson, Pierre ;

Hildebrandt, Niko .

ACS SENSORS, 2019, 4 (10) :2786-2793

[7]

Gorgannezhad L, 2018, LAB CHIP, V18, P1174, DOI [10.1039/c8lc00100f, 10.1039/C8LC00100F]

[8] Autonomous dynamic control of DNA nanostructure self-assembly [J].

Green, Leopold N. ;

Subramanian, Hari K. K. ;

Mardanlou, Vahid ;

Kim, Jongmin ;

Hariadi, Rizal F. ;

Franco, Elisa .

NATURE CHEMISTRY, 2019, 11 (06) :510-520

[9] Core-shell ZnO@C:N hybrids derived from MOFs as long-cycling anodes for lithium ion batteries [J].

Guo, Yichuan ;

Wang, Zhaoying ;

Lu, Xinsheng ;

Lu, Jianguo ;

Rabia, Khatoon ;

Chen, Hongwen ;

Hu, Rui ;

Tang, Haichao ;

Zhang, Qinghua ;

Li, Zhoupeng .

CHEMICAL COMMUNICATIONS, 2020, 56 (13) :1980-1983

[10] Target-driven rolling walker based electrochemical biosensor for ultrasensitive detection of circulating tumor DNA using doxorubicin@tetrahedron-Au tags [J].

Li, Dandan ;

Xu, Yixin ;

Fan, Lu ;

Shen, Bo ;

Ding, Xiaojuan ;

Yuan, Rui ;

Li, Xinmin ;

Chen, Weixian .

BIOSENSORS & BIOELECTRONICS, 2020, 148

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