IBIS - A tool for automated sequential assignment of protein spectra from triple resonance experiments

被引：46

作者：

Hyberts, SG ^{[1
]}

Wagner, G ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 2003年 / 26卷 / 04期

关键词：

automated assignments; backbone assignments; NMR; protein structure; triple resonance experiments;

D O I：

10.1023/A:1024078926886

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a tool for computer-assisted assignments of protein NMR spectra from triple resonance data. The program is designed to resemble established manual assignment procedures as closely as possible. IBIS exports its results in XEASY format. Thus, using IBIS the operator has continuous visual and accounting control over the progress of the assignment procedure. IBIS achieves complete assignments for those residues that exhibit sequential triple resonance connectivities within a few hours or days.

引用

页码：335 / 344

页数：10

共 31 条

[1] THE PROGRAM XEASY FOR COMPUTER-SUPPORTED NMR SPECTRAL-ANALYSIS OF BIOLOGICAL MACROMOLECULES [J].

BARTELS, C ;

XIA, TH ;

BILLETER, M ;

GUNTERT, P ;

WUTHRICH, K .

JOURNAL OF BIOMOLECULAR NMR, 1995, 6 (01) :1-10

[2] Amino-acid-type identification for deuterated proteins with a beta-carbon-edited HNCOCACB experiment [J].

Dotsch, V ;

Matsuo, H ;

Wagner, G .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1996, 112 (01) :95-100

[3]

ECCLES C, 1991, Journal of Biomolecular NMR, V1, P111, DOI 10.1007/BF01877224

[4] AN AUTOMATED PROCEDURE FOR THE ASSIGNMENT OF PROTEIN (HN)-H-1,N-15, C-13(ALPHA), H-1(ALPHA), C-13(BETA) AND H-1(BETA) RESONANCES [J].

FRIEDRICHS, MS ;

MUELLER, L ;

WITTEKIND, M .

JOURNAL OF BIOMOLECULAR NMR, 1994, 4 (05) :703-726

[5]

HARE BJ, 1994, J BIOMOL NMR, V4, P35

[6] A NOVEL-APPROACH FOR SEQUENTIAL ASSIGNMENT OF H-1, C-13, AND N-15 SPECTRA OF LARGER PROTEINS - HETERONUCLEAR TRIPLE-RESONANCE 3-DIMENSIONAL NMR-SPECTROSCOPY - APPLICATION TO CALMODULIN [J].

IKURA, M ;

KAY, LE ;

BAX, A .

BIOCHEMISTRY, 1990, 29 (19) :4659-4667

[7] NMR VIEW - A COMPUTER-PROGRAM FOR THE VISUALIZATION AND ANALYSIS OF NMR DATA [J].

JOHNSON, BA ;

BLEVINS, RA .

JOURNAL OF BIOMOLECULAR NMR, 1994, 4 (05) :603-614

[8] 3-DIMENSIONAL TRIPLE-RESONANCE NMR-SPECTROSCOPY OF ISOTOPICALLY ENRICHED PROTEINS [J].

KAY, LE ;

IKURA, M ;

TSCHUDIN, R ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1990, 89 (03) :496-514

[9] PROTEIN 3-DIMENSIONAL STRUCTURE DETERMINATION AND SEQUENCE-SPECIFIC ASSIGNMENT OF C-13-SEPARATED AND N-15-SEPARATED NOE DATA - A NOVEL REAL-SPACE AB-INITIO APPROACH [J].

KRAULIS, PJ .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (04) :696-718

[10] Automated backbone assignment of labeled proteins using the threshold accepting algorithm [J].

Leutner, M ;

Gschwind, RM ;

Liermann, J ;

Schwarz, C ;

Gemmecker, G ;

Kessler, H .

JOURNAL OF BIOMOLECULAR NMR, 1998, 11 (01) :31-43

← 1 2 3 4 →