Stalk formation as a function of lipid composition studied by X-ray reflectivity

被引：14

作者：

Khattari, Ziad ^{[1
]}

Koehler, Sebastian ^{[2
]}

Xu, Yihui ^{[2
]}

Aeffner, Sebastian ^{[2
]}

Salditt, Tim ^{[2
]}

机构：

[1] Hashemite Univ, Dept Phys, Zarqa 13115, Jordan

[2] Univ Gottingen, Inst Rontgenphys, D-37077 Gottingen, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2015年 / 1848卷 / 01期

关键词：

Membrane fusion; Stalk structure; Multi-component lipid bilayer; X-ray reflectivity; MEMBRANE-FUSION; PHASE-TRANSITIONS; BILAYER STRUCTURE; HYDRATION FORCES; CHOLESTEROL; MECHANISM; DIACYLGLYCEROL; MODEL; SPHINGOMYELIN; ORGANIZATION;

D O I：

10.1016/j.bbamem.2014.08.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the structure and interaction of solid-supported multilamellar phospholipid bilayers in view of stalk formation as model systems for membrane fusion. The multi-component bilayers were composed of ternary and quaternary mixtures, containing phosphatidylcholines, phosphatidylethanolamines, sphingomyelin, cholesterol, diacylglycerol, and phosphatidylinositol. Analysis of the obtained electron density profiles and the pressure-distance curves reveals systematic changes in structure and hydration repulsion. The osmotic pressure needed to induce stalk formation at the transition from the fluid lamellar to the rhombohedral phase indicates how membrane fusion properties are modified by bilayer composition. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：41 / 50

页数：10

共 61 条

[1] Membrane fusion intermediates and the effect of cholesterol: An in-house X-ray scattering study [J].