Analysis of the murine Hoxa-9 cDNA:: an alternatively spliced transcript encodes a truncated protein lacking the homeodomain

被引:26
作者
Fujimoto, S
Araki, K
Chisaka, O
Araki, M
Takagi, K
Yamamura, K
机构
[1] Kumamoto Univ, Sch Med, Inst Mol Embryol & Genet, Dept Dev Genet, Kumamoto 862, Japan
[2] Kumamoto Univ, Sch Med, Dept Orthopaed Surg, Kumamoto 862, Japan
[3] Kyoto Univ, Fac Sci, Dept Biophys, Kyoto 60601, Japan
[4] Kumamoto Univ, Gene Technol Ctr, Kumamoto 862, Japan
基金
日本科学技术振兴机构;
关键词
RT-PCR; primer extension; homeobox; mouse;
D O I
10.1016/S0378-1119(98)00014-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Hoxa-9 is one of the homeo box (Hox) genes exhibiting similarity to the Drosophila Abdominal B gene. So far, only partial nucleotide sequences have been reported for mouse Hoxa-9 cDNA (Rubin et al., (1987) Mol. Cell. Biol. 7, 3836-3841). Here, we have determined the nucleotide sequence of the 5'-region of mouse Hoxa-9 cDNA and its genomic structure. Mouse Hoxa-9 cDNA contains a complete ORF encoding a protein of 271 aa exhibiting 96.7% identity to its human counterpart. Interestingly, an alternatively spliced transcript (Hoxa-9T) was identified by RT-PCR. Sequence analysis revealed that 173 bp within the Hoxa-9 ORF was missing from the Hoxa-9T cDNA. This additional splicing would potentially result in a frameshift, leading to the production of a truncated protein lacking the homeobox. Northern blot analysis revealed that the probe containing the homeodomain hybridized to two major transcripts (2.5 and 1.9 kb) in the trunk region of 12.5 dpc embryos, and adult kidney and large intestine. On the other hand, the probe containing the additional intron detected only 2.5 kb transcript in the same tissues, indicating that 1.9 kb transcript corresponds to Hoxa-9T mRNA. We have also determined the transcriptional start site of Hoxa-9T. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:77 / 85
页数:9
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