A Highly Active DNA Polymerase with a Fluorous Core

被引：35

作者：

Holzberger, Bastian

Rubini, Marina

Moeller, Heiko M.

Marx, Andreas ^{[1
]}

机构：

[1] Univ Konstanz, Dept Chem, D-78457 Constance, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 07期

关键词：

DNA polymerases; NMR spectroscopy; non-natural amino acids; protein engineering; trifluoromethionine; FINGERS SUBDOMAIN; AMINO-ACIDS; PROTEINS; EVOLUTION; FIDELITY;

D O I：

10.1002/anie.200905978

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) A multifluorinated DNA polymerase with all 14 methionine residues (Met; red in the structure) globally replaced by the non-natural amino acid trifluoromethionine (TFM) exhibits enzymatic activity and selectivity similar to the wild type. The fluorinated enzyme serves as a 19F NMR probe, and despite its size of 63 kDa, individual 19F resonances allow the study of enzyme dynamics during DNA synthesis by NMR spectroscopy. © 2010 Wiley-VCH Verlag GmbH &. Co. KGaA.

引用

页码：1324 / 1327

页数：4

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