Bone Targeted Delivery of SDF-1 via Alendronate Functionalized Nanoparticles in Guiding Stem Cell Migration

被引：58

作者：

Chen, Qingchang ^{[1
]}

Zheng, Chuping ^{[1
,3
]}

Li, Yanqun ^{[1
]}

Bian, Shaoquan ^{[1
]}

Pan, Haobo ^{[1
]}

Zhao, Xiaoli ^{[1
]}

Lu, William W. ^{[2
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Res Ctr Human Tissues & Organs Degenerat, Shenzhen 518055, Peoples R China

[2] Univ Hong Kong, Dept Orthopaed & Traumatol, 21 Sassoon Rd, Hong Kong 999077, Hong Kong, Peoples R China

[3] Guangzhou Med Univ, Sch Pharmaceut Sci, Guangzhou 511436, Guangdong, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2018年 / 10卷 / 28期

基金：

中国国家自然科学基金;

关键词：

bone targeting; gene delivery; stem cell homing; SDF-1; osteoporosis therapy; POLY(ETHYLENE GLYCOL); MESENCHYMAL CELLS; GENE-TRANSFER; FACTOR-I; RESORPTION; THERAPY; PEPTIDE; REPAIR; TRANSPLANTATION; TRAFFICKING;

D O I：

10.1021/acsami.8b08606

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Stem cells are well-known for their great capacity for tissue regeneration. This provides a promising source for cell-based therapies in treating various bone degenerative disorders. However, the major hurdles for their application in transplantation are the poor bone marrow homing and engraftment efficiencies. Stromal cell-derived factor 1 (SDF-1) has been identified as a major stem cell homing factor. With the aims of bone targeted SDF-1 delivery and regulating MSCs migration, alendronate modified liposomal nanoparticles (Aln-Lipo) carrying SDF-1 gene were developed in this study. Alendronate modification significantly increased the mineral binding affinity of liposomes, and facilitated the gene delivery to osteoblastic cells. Up-regulated SDF-1 expression in osteoblasts triggered MSCs migration. Systemic infusion of Aln-Lipo-SDF-1 with fluorescence labeling in mice showed the accumulation in osseous tissue by biophotonic imaging. Corresponding to the delivered SDF-1, the transplanted GFP+ MSCs were attracted to bone marrow and contributed to bone regeneration. This study may provide a useful technique in regulating stem cell migration.

引用

页码：23700 / 23710

页数：11

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