Effect of mating delay on the ovary of Melipona quadrifasciata anthidioides (Hymenoptera: Apidae) queens

被引:12
作者
de Souza, Edmilson Amaral
Neves, Clovis Andrade
de Oliveira Campos, Lucio Antonio
Zanuncio, Jose Cola
Serrao, Jose Eduardo
机构
[1] Univ Fed Vicosa, Dept Biol Geral, BR-35570000 Vicosa, MG, Brazil
[2] Univ Fed Vicosa, Dept Biol Anim, Vicosa, MG, Brazil
关键词
ovary degeneration; virgin queen; mating; oosorption;
D O I
10.1016/j.micron.2006.08.005
中图分类号
TH742 [显微镜];
学科分类号
摘要
The effect of mating delay on the ovary structure of virgin queens of Melipona quadrifasciata anthidioides (Hymenoptera: Apidae) was investigated. Virgin queens 15, 20, 25 and 30 days old were dissected to analyze their degree of ovary degeneration. To verify whether the degeneration would cause permanent sterility, virgin queens of the same ages (15, 20, 25 and 30 days) were mated and accompanied for at least 14 days to verify whether there was physogastry and then dissected. The ovaries were analyzed by histology, histochemical tests and TUNEL to verify programmed cell death. The results showed that mating delay interrupted oogenesis preventing vitellogenesis. Mating delay results in ovary degeneration which increased with queen age. However, even when there was ovariole degeneration, 25-day-old virgin queens after mating presented normal ovariole activation. (c) 2006 Published by Elsevier Ltd.
引用
收藏
页码:471 / 477
页数:7
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