Detection of Organophosphate Pesticide Using Polyaniline and Carbon Nanotubes Composite Based on Acetylcholinesterase Inhibition

被引：13
作者：
Chen, Daiqin [1 ]
Chen, Chuanhuizi [1 ]
Du, Dan [1 ]
机构：
[1] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2010年 / 10卷 / 09期
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词：
Acetylcholinesterase; PANI-MWCNTs Composite Film; Malathion; SCREEN-PRINTED BIOSENSOR; ARRAYS; CELLS;
D O I：
10.1166/jnn.2010.2477
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity.
引用
收藏
页码：5662 / 5666
页数：5
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