Epitope-blocking enzyme-linked immunosorbent assays for detection of West Nile virus antibodies in domestic mammals

被引：91
作者：
Blitvich, BJ
Bowen, RA
Marlenee, NL
Hall, RA
Bunning, ML
Beaty, BJ [1 ]
机构：
[1] Colorado State Univ, Arthropod Borne & Infect Dis Lab, Dept Microbiol Immunol & Parasitol, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Anim Reprod & Biotechnol Lab, Equine Ctr, Ft Collins, CO 80523 USA
[3] Univ Queensland, Dept Microbiol & Parasitol, St Lucia, Qld 4072, Australia
[4] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Ft Collins, CO 80522 USA
[5] USAF, Off Surg Gen, Washington, DC 20332 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 06期
关键词：
D O I：
10.1128/JCM.41.6.2676-2679.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
引用
收藏
页码：2676 / 2679
页数：4
相关论文
共 17 条
  • [1] Structures of immature flavivirus particles
    Zhang, Y
    Corver, J
    Chipman, PR
    Zhang, W
    Pletnev, SV
    Sedlak, D
    Baker, TS
    Strauss, JH
    Kuhn, RJ
    Rossmann, MG
    [J]. EMBO JOURNAL, 2003, 22 (11) : 2604 - 2613
  • [2] Beaty BJ., 1989, Diagnostic procedures for viral, rickettsial and chlamydial infections, P797
  • [3] Epitope-blocking enzyme-linked immunosorbent assays for the detection of serum antibodies to West Nile virus in multiple avian species
    Blitvich, BJ
    Marlenee, NL
    Hall, RA
    Calisher, CH
    Bowen, RA
    Roehrig, JT
    Komar, N
    Langevin, SA
    Beaty, BJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) : 1041 - 1047
  • [4] CASALS J, 1973, SEROLOGICAL EPIDEMIO, P99
  • [5] West Nile virus recombinant DNA vaccine protects mouse and horse from virus challenge and expresses in vitro a noninfectious recombinant antigen that can be used in enzyme-linked immunosorbent assays
    Davis, BS
    Chang, GJJ
    Cropp, B
    Roehrig, JT
    Martin, DA
    Mitchell, CJ
    Bowen, R
    Bunning, ML
    [J]. JOURNAL OF VIROLOGY, 2001, 75 (09) : 4040 - 4047
  • [6] Eidson M, 2001, EMERG INFECT DIS, V7, P615
  • [7] The West Nile virus: its recent emergence in North America
    Garmendia, AE
    Van Kruiningen, HJ
    French, RA
    [J]. MICROBES AND INFECTION, 2001, 3 (03) : 223 - 229
  • [8] West Nile virus in the United States: Guidelines for detection, prevention, and control
    Gubler, DJ
    Campbell, GL
    Nasci, R
    Komar, N
    Petersen, L
    Roehrig, JT
    [J]. VIRAL IMMUNOLOGY, 2000, 13 (04) : 469 - 475
  • [9] IMMUNODOMINANT EPITOPES ON THE NS1 PROTEIN OF MVE AND KUN VIRUSES SERVE AS TARGETS FOR A BLOCKING ELISA TO DETECT VIRUS-SPECIFIC ANTIBODIES IN SENTINEL ANIMAL SERUM
    HALL, RA
    BROOM, AK
    HARTNETT, AC
    HOWARD, MJ
    MACKENZIE, JS
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1995, 51 (2-3) : 201 - 210
  • [10] EPITOPE ANALYSIS OF THE ENVELOPE AND NONSTRUCTURAL GLYCOPROTEINS OF MURRAY VALLEY ENCEPHALITIS-VIRUS
    HALL, RA
    KAY, BH
    BURGESS, GW
    CLANCY, P
    FANNING, ID
    [J]. JOURNAL OF GENERAL VIROLOGY, 1990, 71 : 2923 - 2930
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