Housekeeping genes; expression levels may change with density of cultured cells

被引：105

作者：

Greer, Samantha

Honeywell, Rice

Geletu, Mulu

Arulanandam, Rozanne

Raptis, Leda ^{[1
]}

机构：

[1] Queens Univ, Dept Microbiol & Immunol, Kingston, ON K7L 3N6, Canada

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 355卷 / 1-2期

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

alpha-tubulin; GAPDH; Hsp90; beta-actin; Gel electrophoresis; Cell confluence; STAT3; ACTIVITY;

D O I：

10.1016/j.jim.2010.02.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Western blotting is a powerful technique to characterize a multitude of cellular proteins. As an internal control, the blots are commonly probed for "housekeeping" gene products. In this communication, we show that cell confluence significantly affects the levels of two such widely used proteins, alpha-tubulin and Glyceraldehyde-3-Phosphate Dehydrogenase. On the other hand the levels of heat-shock protein-90 and beta-actin remained unchanged at a wide range of cell densities, making these proteins into more reliable loading controls. (C) 2010 Elsevier B.V. All rights reserved.

引用

收藏

页码：76 / 79

页数：4

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