Rational design of hybridization chain reaction monomers for robust signal amplification

被引：81

作者：

Ang, Yan Shan ^{[1
]}

Yung, Lin-Yue Lanry ^{[1
]}

机构：

[1] Natl Univ Singapore, Chem & Biomol Engn, 4 Engn Dr 4, Singapore 117585, Singapore

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 22期

关键词：

NUCLEIC-ACID CIRCUITS; DNA DETECTION; ULTRASENSITIVE DETECTION; MOLECULAR BEACONS; PROTEIN-DETECTION; ASSAY; NANOTECHNOLOGY; COMPLEX; CELLS; GAIN;

D O I：

10.1039/c5cc08907g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We established four-point guidelines for the sequence design of hairpin monomers in hybridization chain reaction (HCR). This enabled greater flexibility to customize specific hairpin sequences for use with the readout platform of interest. Using shorter hairpin stem length, a one-pot signal amplification system was demonstrated by incorporating distance-sensitive Forster resonance energy transfer (FRET) readout.

引用

页码：4219 / 4222

页数：4

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