Newly identified allatostatin Bs and their receptor in the two-spotted cricket, Gryllus bimaculatus

被引：11
作者：
Tsukamoto, Yusuke [1 ,2 ]
Nagata, Shinji [1 ]
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwanoha 5-1-5, Kashiwa, Chiba 2778567, Japan
[2] JSPS, Tokyo, Japan
来源：
PEPTIDES | 2016年 / 80卷
关键词：
Allatostatin-B; Ca2+ imaging; Gryllus bimaculatus; Receptor; MELANOGASTER SEX-PEPTIDE; VENTRAL NERVE CORD; JUVENILE-HORMONE; MYOINHIBITORY PEPTIDES; DROSOPHILA; NEUROPEPTIDES; EXPRESSION; ENSIFERA; GENE; MIPS;
D O I：
10.1016/j.peptides.2016.03.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA encoding allatostatin Bs (ASTBs) containing the W(X)(6)W motif was identified using a database generated by a next generation sequencer (NGS) in the two-spotted cricket, Gryllus bimaculatus. The contig sequence revealed the presence of five novel putative ASTBs (GbASTBs) in addition to GbASTBs previously identified in G. bimaculatus. MALDI-TOF MS analyses revealed the presence of these novel and previously identified GbASTBs with three missing GbASTBs. We also identified a cDNA encoding G. bimaculatus GbASTB receptor (GbASTBR) in the NGS data. Phylogenetic analysis demonstrated that this receptor was highly conserved with other insect ASTBR5, including the sex peptide receptor of Drosophila melanogaster. Calcium imaging analyses indicated that the GbASTBR heterologously expressed in HEK293 cells exhibited responses to all identified GbASTBs at a concentration range of 10(-10)-10(-5) M. (C) 2016 Elsevier Inc. All rights reserved.
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收藏
页码：25 / 31
页数：7
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