An experiment was established in autumn 1992 to study the interactive effects of multiple weed species competition in winter wheat. Galium aparine, Matricaria perforata and Papaver rhoeas were grown singly and as pairs of species in wheat. P. rhoeas produced most biomass both singly and in mixture resulting in greatest crop biomass and yield reductions. In mixture, P. rhoeas had the greatest influence in depressing the biomass of the associated species. The effects of weeds in mixture on crop biomass were additive in May, but at harvest, yield reductions from weeds in mixture were only slightly greater than from single species.