Partial purification and characterization of a Gibberellin-binding protein from seedlings of Azukia angularis

被引:17
作者
Nakajima, M [1 ]
Takita, K [1 ]
Wada, H [1 ]
Mihara, K [1 ]
Hasegawa, M [1 ]
Yamaguchi, I [1 ]
Murofushi, N [1 ]
机构
[1] Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 113, Japan
关键词
D O I
10.1006/bbrc.1997.7896
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Little is known about mechanisms for the perception of gibberellin (GA), a phytohormone, by plants. A GA-binding protein (GBP) with receptor-like characteristics was partially purified from azuki bean (Azukia angularis) seedlings. Gibberellins that caused much elongation of azuki epicotyl sections showed strong binding affinity for the GBP, and their biosynthetic precursors and inactive metabolites had weak affinity for it. The apparent K-d value for GA(4) was calculated to be 7 x 10(-10) M from Scatchard plots. The molecular weight of the GBP in the native state was estimated to be 25,000 by high-performance liquid chromatography with a gel-permeation column. The GBP may be one of the receptor candidates for GAs. (C) 1997 Academic Press.
引用
收藏
页码:782 / 786
页数:5
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