Effect of glutathione-modulating compounds on hydrogen peroxide-induced cytotoxicity in human glioblastoma and glioma cell lines

被引：23

作者：

Iida, M

Sunaga, S

Hirota, N

Kuribayashi, N

Sakagami, H

Takeda, M

Matsumoto, K

机构：

[1] Showa Univ, Sch Med, Dept Neurosurg, Shinagawa Ku, Tokyo 142, Japan

[2] Showa Univ, Sch Med, Dept Biochem, Tokyo 142, Japan

来源：

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 1997年 / 123卷 / 11-12期

关键词：

glioblastoma; glioma; hydrogen peroxide; glutathione;

D O I：

10.1007/s004320050115

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The relation between the intracellular glutathione (GSH) concentration and hydrogen-peroxide(H2O2)-induced cytotoxicity was investigated. The intracellular GSH concentration in human glioblastoma (T98G, U87MG) and glioma (KGlC) cell lines was one or two orders of magnitude higher than that in a human myelogenous leukemic cell line (HL-60), which showed higher sensitivity to H2O2. Pretreatment of these cell lines with L-buthionine-[S,R]-sulfoximine, which significantly reduced the intracellular GSH concentration, increased their sensitivity against H2O2, whereas pretreatment with N-acetyl-L-cysteine, which did not significantly change the intracellular GSH concentration, only marginally protected the cells from the cytotoxic effect of H2O2. The results suggest that drug sensitivity of tumor cells can be modified by glutathione-modulating compounds.

引用

页码：619 / 622

页数：4

共 16 条

[1] THE ANTIOXIDANT ACTION OF N-ACETYLCYSTEINE - ITS REACTION WITH HYDROGEN-PEROXIDE, HYDROXYL RADICAL, SUPEROXIDE, AND HYPOCHLOROUS ACID [J].