Fluorescent Ribonucleoside as a FRET Acceptor for Tryptophan in Native Proteins

被引：52

作者：

Xie, Yun ^{[1
]}

Maxson, Tucker ^{[1
]}

Tor, Yitzhak ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 34期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

AMINO-ACIDS; HIV-1;

D O I：

10.1021/ja105244t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new fluorescent ribonucleoside analogue, containing 5-aminoquinazoline-2,4(1H,3H)-dione, acts as a Forster resonance energy transfer acceptor for tryptophan (R-0 = 22 angstrom) and displays visible emission (440 nm). As tryptophan is frequently found at or near the recognition domains of RNA binding proteins, this FRET pair facilitates the study of RNA binding to native proteins and peptides, which is demonstrated here for the HIV-1 Rev association with the Rev Response Element (RRE).

引用

页码：11896 / 11897

页数：2

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