Molecular identification of Chilli leaf curl India virus along with betasatellite molecule causing leaf curl disease of menthol mint (Mentha arvensis var. Kosi) in India

被引:6
|
作者
Saeed, Sana Tabanda [1 ]
Kumar, B. [2 ]
Shasany, A. K. [3 ]
Samad, A. [1 ]
机构
[1] CSIR Cent Inst Med & Aromat Plants, Dept Plant Pathol, Lucknow 226015, Uttar Pradesh, India
[2] CSIR Cent Inst Med & Aromat Plants, Genet & Plant Breeding Div, Lucknow 226015, Uttar Pradesh, India
[3] CSIR Cent Inst Med & Aromat Plants, Dept Biotechnol, Lucknow 226015, Uttar Pradesh, India
关键词
Begomovirus; Betasatellite; Chilli leaf curl disease; Mentha arvensis; PCR-MEDIATED AMPLIFICATION; UNIVERSAL PRIMERS; DNA-BETA; GEMINIVIRUSES;
D O I
10.1007/s10327-017-0730-y
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Whitefly-transmitted begomovirus induces leaf curling and stunting of Mentha arvensis (Kosi) and is responsible for heavy economic losses in and around Lucknow, India. The complete genome was amplified, sequenced and assembled as 2759 bp long, with 1376 bp betasatellite molecule. Because no DNA-B amplicon was detected, the virus was classified as "Old World begomovirus" with the characteristic seven ORFs; two ORFs (AV1, AV2) in the V-sense and five ORFs (AC1-AC5) in the C-sense. BLASTn and phylogenetic analysis showed highest similarity (98%) with chilli leaf curl India virus (ChiLCINV). This is the first report of ChiLCINV infection in M. arvensis (Kosi) from India.
引用
收藏
页码:333 / 336
页数:4
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