First record of isolation and characterization of Vibrio sinaloensis from diseased orange-spotted grouper Epinephelus coioides

被引:0
作者
Zhang, Xue [1 ]
Sun, Jingfeng [1 ]
Zhu, Yonglong [1 ]
Han, Zhuoran [1 ]
Hu, Xiucai [1 ]
Lv, Aijun [1 ]
Guo, Yongjun [1 ]
机构
[1] Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300380, Peoples R China
基金
中国国家自然科学基金;
关键词
Epinephelus coioides; Vibrio sinaloensis; Bacterial identification; Histopathology; HARVEYI;
D O I
10.3354/dao03665
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A dominant bacterium, ZYL-12, isolated from the liver of a diseased orange-spotted grouper Epinephelus coioides, was identified as Vibrio sinaloensis, based on phenotypic and molecular analysis. The median lethal dosage of ZYL-12 was calculated as 1.6 x 10(5) CFU g(-1) fish weight. The infection experiment indicated that ZYL-12 caused noticeable histological lesions to the liver, kidney and spleen of the fish. Growth characteristics showed that ZYL-12 possessed strong environmental adaptability. This note is the first report about the pathogenicity of V. sinaloensis isolated from diseased fish.
引用
收藏
页码:71 / 76
页数:6
相关论文
共 17 条
[1]  
Buchanan RE, 1974, BERGEYS MANUAL DETER, V8th
[2]   Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper ( Epinephelus lanceolatus x Epinephelus fuscoguttatus) [J].
Bunlipatanon, Paiboon ;
U-taynapun, Kittichon .
AQUACULTURE RESEARCH, 2017, 48 (04) :1711-1723
[3]  
Chatterjee S., 2012, J MAR SCI RES DEV, VS1, P002, DOI [10.4172/2155-9910.S1-002, DOI 10.4172/2155-9910.S1-002]
[4]   Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio) [J].
Chen, Feng ;
Sun, Jingfeng ;
Han, Zhuoran ;
Yang, Xijun ;
Xian, Jian-an ;
Lv, Aijun ;
Hu, Xiucai ;
Shi, Hongyue .
FRONTIERS IN MICROBIOLOGY, 2019, 10
[5]   Vibrio variabilis sp nov and Vibrio maritimus sp nov., isolated from Palythoa caribaeorum [J].
Chimetto, Luciane A. ;
Cleenwerck, Ilse ;
Moreira, Ana Paula B. ;
Brocchi, Marcelo ;
Willems, Anne ;
De Vos, Paul ;
Thompson, Fabiano L. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011, 61 :3009-3015
[6]  
Flores-Miranda MD, 2012, REV BIOL TROP, V60, P567
[7]   Vibrio japonicus sp nov., a novel member of the Nereis clade in the genus Vibrio isolated from the coast of Japan [J].
Doi, Hiroyasu ;
Osawa, Ikuko ;
Adachi, Hayamitsu ;
Kawada, Manabu .
PLOS ONE, 2017, 12 (02)
[8]   THE MEDIAN LETHAL DOSE AND ITS ESTIMATION [J].
FINNEY, DJ .
ARCHIVES OF TOXICOLOGY, 1985, 56 (04) :215-218
[9]   Vibrio sinaloensis sp nov., isolated from the spotted rose snapper, Lutjanus guttatus Steindachner, 1869 [J].
Gomez-Gil, Bruno ;
Fajer-Avila, Emma ;
Pascual, Javier ;
Macian, M. Carmen ;
Pujalte, Maria J. ;
Garay, Esperanza ;
Roque, Ana .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008, 58 :1621-1624
[10]   Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Gunther [J].
Han, Zhuoran ;
Sun, Jingfeng ;
Lv, Aijun ;
Sung, YeongYik ;
Shi, Hongyue ;
Hu, Xiucai ;
Xing, Kezhi .
AQUACULTURE, 2017, 468 :356-362