A homolog of the defender against apoptotic death gene (DAD1) in senescing gladiolus petals is down-regulated prior to the onset of programmed cell death

被引:35
|
作者
Yamada, T [1 ]
Takatsu, Y
Kasumi, M
Marubashi, W
Ichimura, K
机构
[1] Natl Agr & Biooriented Res Org, Natl Inst Floricultural Sci, Ibaraki 3058519, Japan
[2] Ibaraki Agr Ctr, Plant Biotechnol Inst, Ibaraki 3190292, Japan
[3] Ibaraki Univ, Sch Agr, Ibaraki 3000393, Japan
关键词
DAD1; gladiolus x grandifora; DNA fragmentation; nuclear fragmentation; petal senescene; programmed cell death;
D O I
10.1016/j.jplph.2004.06.005
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We isolated a homolog of the potential anti-apoptotic gene, defender against apoptotic death (DAD1) from gladiolus petals as full-length cDNA (GlDAD1), and investigated the relationship between its expression and the execution processes of programmed cell death (PCD) in senescing petals. RNA get blotting showed that GlDAD1 expression in petals was drastically reduced, considerably before the first visible senescence symptom (petal witting). A few days after down-regulation GlDAD1 expression, DNA and nuclear fragmentation were observed, both specific for the execution phase of PCD. (C) 2004 Elsevier GmbH. All rights reserved.
引用
收藏
页码:1281 / 1283
页数:3
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