Expression and export: recombinant protein production systems for Aspergillus

被引：116

作者：

Fleissner, Andre ^{[3
]}

Dersch, Petra ^{[1
,2
]}

机构：

[1] Helmholtz Zentrum Infekt Forsch, D-38124 Braunschweig, Germany

[2] Helmholtz Ctr Infect Res, Dept Mol Infect Biol, D-38124 Braunschweig, Germany

[3] Tech Univ Carolo Wilhelmina Braunschweig, Inst Genet, D-38106 Braunschweig, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 87卷 / 04期

关键词：

Aspergillus niger; Gene expression; Inducible promoters; Cloning vectors; Protein production; Secretion systems; HETEROLOGOUS GENE-EXPRESSION; CHAIN ANTIBODY FRAGMENT; LIPASE-ENCODING GENE; NIGER VAR. AWAMORI; FILAMENTOUS FUNGI; FUSION PROTEIN; MULTIPLE COPIES; MEDIATED TRANSFORMATION; ENDOPLASMIC-RETICULUM; INDUCIBLE EXPRESSION;

D O I：

10.1007/s00253-010-2672-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several Aspergillus species, in particular Aspergillus niger and Aspergillus oryzae, are widely used as protein production hosts in various biotechnological applications. In order to improve the expression and secretion of recombinant proteins in these filamentous fungi, several novel genetic engineering strategies have been developed in recent years. This review describes state-of-the-art genetic manipulation technologies used for strain improvement, as well as recent advances in designing the most appropriate engineering strategy for a particular protein production process. Furthermore, current developments in identifying bottlenecks in the protein production and secretion pathways are described and novel approaches to overcome these limitations are introduced. An appropriate combination of expression vectors and optimized host strains will provide cell factories customized for each production process and expand the great potential of Aspergilli as biotechnology workhorses to more complex multi-step industrial applications.

引用

页码：1255 / 1270

页数：16

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