Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS

被引：22

作者：

Chen, Shey-Ying ^{[1
]}

Lee, Hao ^{[2
]}

Teng, Shih-Hua ^{[3
]}

Wang, Xiao-Mei ^{[4
]}

Lee, Tai-Fen ^{[2
]}

Huang, Yu-Chung ^{[2
,5
]}

Liao, Chun-Hsing ^{[5
]}

Teng, Lee-Jene ^{[2
,4
]}

Hsueh, Po-Ren ^{[2
,6
]}

机构：

[1] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Emergency Med, Coll Med, Taipei, Taiwan

[2] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Dept Lab Med, Coll Med, Taipei, Taiwan

[3] Bruker Taiwan Co Ltd, Taipei, Taiwan

[4] Natl Taiwan Univ, Dept Med Genet, Coll Med, Taipei, Taiwan

[5] Far Eastern Mem Hosp, Dept Internal Med, New Taipei, Taiwan

[6] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Coll Med, Dept Internal Med, Taipei, Taiwan

来源：

FUTURE MICROBIOLOGY | 2018年 / 13卷 / 09期

关键词：

Bruker Biotyper MALDI-TOF MS; classification model; matrix-assisted laser desorption/ionization-time of flight mass spectrometry; Staphylococcus argenteus; Staphylococcus aureus; FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; BRUKER BIOTYPER; CLONAL COMPLEX; IDENTIFICATION; SYSTEM; EPIDEMIOLOGY; NOCARDIA;

D O I：

10.2217/fmb-2018-0015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. Conclusion: The classification model could accurately differentiate S. argenteus from MSSA.

引用

页码：997 / 1006

页数：10

共 32 条

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