Development of surface plasmon resonance imaging biosensors for detection of ubiquitin carboxyl-terminal hydrolase L1

被引：38

作者：

Sankiewicz, Anna ^{[1
]}

Laudanski, Piotr ^{[2
]}

Romanowicz, Lech ^{[3
]}

Hermanowicz, Adam ^{[4
]}

Roszkowska-Jakimiec, Wieslawa ^{[5
]}

Debek, Wojciech ^{[4
]}

Gorodkiewicz, Ewa ^{[1
]}

机构：

[1] Univ Bialystok, Inst Chem, Dept Electrochem, PL-15443 Bialystok, Poland

[2] Med Univ Bialystok, Dept Perinatol, PL-15276 Bialystok, Poland

[3] Med Univ Bialystok, Dept Med Biochem, PL-15089 Bialystok, Poland

[4] Med Univ Bialystok, Dept Pediat Surg, PL-15274 Bialystok, Poland

[5] Med Univ Bialystok, Dept Analyt Chem, PL-15230 Bialystok, Poland

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 469卷

关键词：

Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1); Surface plasmon resonance imaging; Biosensor; PARKINSONS-DISEASE; PROTEASOME PATHWAY; SENSOR DEVELOPMENT; POTENT INHIBITOR; BIOMARKER; UCH-L1; CANCER; EXPRESSION; SURVIVAL; PGP9.5;

D O I：

10.1016/j.ab.2014.09.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a new method for highly selective determination of the ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) concentration using a surface plasmon resonance imaging (SPRI) technique and two different biosensors. UCH-L1 was captured from a solution by immobilized specific rabbit monoclonal antibody or specific LDN-57444 inhibitor due to formation of receptor-UCH-L1 complex on the biosensor surface. The analytically useful dynamic response range of both biosensors is between 0.1 and 2.5 ng/ml. The detection limit is 0.06 ng/ml for the biosensor with antibody and 0.08 ng/ml for the biosensor with inhibitor. Biosensors based on both antibody and inhibitor were found to be suitable for quantitative determination of the UCH-L1 and exhibit good tolerance to the potential interferents. Both biosensors gave comparable results in the range of 0 to 0.20 ng/ml for plasma samples and 0.30 to 0.49 ng/ml for cerebrospinal fluid samples. To validate the new methods, comparative determination of UCH-L1 by the commercial enzyme-linked immunosorbent assay (ELISA) kit was performed. In general, in terms of UCH-L1 concentration, a good correlation between SPRI and ELISA was found. The developed biosensors can be used successfully for the determination of UCH-L1 in body fluids. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：4 / 11

页数：8