Concepts for nanoscale resolution in fluorescence microscopy

被引：210
作者：
Hell, SW [1 ]
Dyba, M [1 ]
Jakobs, S [1 ]
机构：
[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37070 Gottingen, Germany
来源：
CURRENT OPINION IN NEUROBIOLOGY | 2004年 / 14卷 / 05期
关键词：
D O I：
10.1016/j.conb.2004.08.015
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Spatio-temporal visualization of cellular structures by fluorescence microscopy has become indispensable in biology. However, the resolution of conventional fluorescence microscopy is limited by diffraction to about 180 nm in the focal plane and to about 500 nm along the optic axis. Recently, concepts have emerged that overcome the diffraction resolution barrier fundamentally. Formed on the basis of reversible saturable optical transitions, these concepts might eventually allow us to investigate hitherto inaccessible details within live cells.
引用
收藏
页码：599 / 609
页数：11
相关论文
共 60 条
  • [1] Abbe E., 1873, Archiv f)r mikroskopische Anatomie, V9, P413, DOI DOI 10.1007/BF02956173
  • [2] Semiconductor clusters, nanocrystals, and quantum dots
    Alivisatos, AP
    [J]. SCIENCE, 1996, 271 (5251) : 933 - 937
  • [3] 4Pi-confocal microscopy of live cells
    Bahlmann, K
    Jakobs, S
    Hell, SW
    [J]. ULTRAMICROSCOPY, 2001, 87 (03) : 155 - 164
  • [4] ENHANCEMENT OF AXIAL RESOLUTION IN FLUORESCENCE MICROSCOPY BY STANDING-WAVE EXCITATION
    BAILEY, B
    FARKAS, DL
    TAYLOR, DL
    LANNI, F
    [J]. NATURE, 1993, 366 (6450) : 44 - 48
  • [5] 3-DIMENSIONAL IMAGE-RESTORATION AND SUPERRESOLUTION IN FLUORESCENCE CONFOCAL MICROSCOPY
    BERTERO, M
    BOCCACCI, P
    BRAKENHOFF, GJ
    MALFANTI, F
    VANDERVOORT, HTM
    [J]. JOURNAL OF MICROSCOPY, 1990, 157 : 3 - 20
  • [6] RESOLUTION IN DIFFRACTION-LIMITED IMAGING, A SINGULAR VALUE ANALYSIS .4. THE CASE OF UNCERTAIN LOCALIZATION OR NON-UNIFORM ILLUMINATION OF THE OBJECT
    BERTERO, M
    DEMOL, C
    PIKE, ER
    WALKER, JG
    [J]. OPTICA ACTA, 1984, 31 (08): : 923 - 946
  • [7] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY
    BETZIG, E
    CHICHESTER, RJ
    [J]. SCIENCE, 1993, 262 (5138) : 1422 - 1425
  • [8] Single sharp spot in fluorescence microscopy of two opposing lenses
    Blanca, CM
    Bewersdorf, J
    Hell, SW
    [J]. APPLIED PHYSICS LETTERS, 2001, 79 (15) : 2321 - 2323
  • [9] Bloembergen N., 1965, NONLINEAR OPTICS
  • [10] Born M., 2002, PRINCIPLES OPTICS
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