Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine: Method Optimisation and Profiling in Healthy Adults upon Short-Term Dietary Intervention

被引：19

作者：

Bravo, Aida Corrillero ^{[1
]}

Aguilera, Maria Nieves Ligero ^{[1
]}

Marziali, Nahuel R. ^{[1
]}

Moritz, Lennart ^{[1
]}

Wingert, Victoria ^{[1
]}

Klotz, Katharina ^{[1
]}

Schumann, Anke ^{[1
,2
]}

Gruenert, Sarah C. ^{[2
]}

Spiekerkoetter, Ute ^{[2
]}

Berger, Urs ^{[1
]}

Lederer, Ann-Kathrin ^{[3
]}

Huber, Roman ^{[3
]}

Hannibal, Luciana ^{[1
]}

机构：

[1] Univ Freiburg, Med Ctr, Fac Med, Dept Gen Pediat Adolescent Med & Neonatol,Lab Cli, D-79106 Freiburg, Germany

[2] Univ Freiburg, Med Ctr, Fac Med, Dept Gen Pediat Adolescent Med & Neonatol, D-79106 Freiburg, Germany

[3] Univ Freiburg, Med Ctr, Fac Med, Ctr Complementary Med,Dept Internal Med 2, D-79106 Freiburg, Germany

来源：

METABOLITES | 2022年 / 12卷 / 05期

关键词：

S-adenosylmethionine; S-adenosylhomocysteine; methionine; cobalamin; folate; homocysteine; targeted metabolomics; inborn errors of metabolism; CEREBROSPINAL-FLUID; METHIONINE; PLASMA; HOMOCYSTEINE; DISEASE; QUANTIFICATION; METABOLITES; DIAGNOSIS; RISK;

D O I：

10.3390/metabo12050373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

S-adenosylmethionine (SAM) is essential for methyl transfer reactions. All SAM is produced de novo via the methionine cycle. The demethylation of SAM produces S-adenosylhomocysteine (SAH), an inhibitor of methyltransferases and the precursor of homocysteine (Hcy). The measurement of SAM and SAH in plasma has value in the diagnosis of inborn errors of metabolism (IEM) and in research to assess methyl group homeostasis. The determination of SAM and SAH is complicated by the instability of SAM under neutral and alkaline conditions and the naturally low concentration of both SAM and SAH in plasma (nM range). Herein, we describe an optimised LC-MS/MS method for the determination of SAM and SAH in plasma, urine, and cells. The method is based on isotopic dilution and employs 20 mu L of plasma or urine, or 500,000 cells, and has an instrumental running time of 5 min. The reference ranges for plasma SAM and SAH in a cohort of 33 healthy individuals (age: 19-60 years old; mean +/- 2 SD) were 120 +/- 36 nM and 21.5 +/- 6.5 nM, respectively, in accordance with independent studies and diagnostic determinations. The method detected abnormal concentrations of SAM and SAH in patients with inborn errors of methyl group metabolism. Plasma and urinary SAM and SAH concentrations were determined for the first time in a randomised controlled trial of 53 healthy adult omnivores (age: 18-60 years old), before and after a 4 week intervention with a vegan or meat-rich diet, and revealed preserved variations of both metabolites and the SAM/SAH index.

引用

页数：21

共 64 条

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