Antibody response after vaccination with antigen-pulsed dendritic cells

被引:2
|
作者
Battaini, F
Besusso, D
Sfondrini, L
Rossini, A
Morelli, D
Tagliabue, E
Menard, S
Balsari, A
机构
[1] Ist Nazl Tumori, Dept Expt Oncol, Mol Targeting Unit, Chair Immunol, I-20133 Milan, Italy
[2] Ist Nazl Tumori, Dept Expt Oncol, Med Lab Unit, I-20133 Milan, Italy
[3] Univ Milan, Inst Pathol, I-20122 Milan, Italy
来源
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 2004年 / 19卷 / 03期
关键词
antigen-presenting cells (APCs); dendritic cells (DCs); HER2; vaccination; antibody response;
D O I
10.1177/172460080401900306
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune system capable of initiating immune responses to antigens. It is also well documented that cancer patients often experience anergy against tumor antigens. In this study we selected the best protocol for inducing the production of antibodies against the HER2 oncoprotein using DCs to overcome anergy. Murine DCs were pulsed in vitro, using different protocols, with recombinant HER2 fused to a human Fc (in order to improve DC antigen uptake) and were used to vaccinate mice. The obtained results indicate that antigen-pulsed DCs can induce an antibody response and that adding CpG after antigen pulsing greatly increases anti-HER2 antibody production.
引用
收藏
页码:213 / 220
页数:8
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