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Probes for the specific detection of Cryptosporidium parvum
被引：15
|作者：
Lindquist, HDA
机构：
[1] Biohazard Assessment Research Branch, Human Exposure Research Division, Natl. Exposure Res. Lab. U.S. E., Cincinnati, OH 45268-1320
关键词：
Cryptosporidium parvum;
ribosomal;
rRNA;
probe;
oocyst;
detection;
D O I：
10.1016/S0043-1354(97)00076-6
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ;
0830 ;
摘要：
A probe set, consisting of two synthetic oligonucleotides each tagged with a fluorescent reporter molecule, has been developed for specific detection of Cryptosporidium parvum. Each probe strand detects ribosomal RNA from a range of isolates of this species, and the combination is designed to allow detection of all isolates for which the rRNA sequence has been published. This represents the first use of 6-carboxyfluorescein phosphoramadite as a label for fluorescent in situ hybridization. Published by Elsevier Science Ltd.
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页码：2668 / 2671
页数：4
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