Study on the toxic effect of Lead(II) ion on Escherichia coli

被引：16

作者：

Peng, Liu ^{[1
]}

Ruan Lifang

Xiao Hongyu

Xi, Li

Zhang Chaocan

机构：

[1] Wuhan Univ Technol, Sch Sci, Wuhan 430070, Peoples R China

[2] Wuhan Univ Technol, Sch Mat Sci & Technol, Wuhan 430070, Peoples R China

[3] Huazhong Agr Univ, Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2007年 / 115卷 / 02期

关键词：

lead; toxic effect; microcalorimetry; inhibition; outer membrane;

D O I：

10.1007/BF02686030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The toxic effects of lead(II) have been studied in Escherichia coli cells. Using microcalorimetric analysis, it was shown that E. coli growth was inhibited in the presence of Pb2+ resulting from damage to the cell membrane and that Pb2+ takes part in the metabolism of cells. Treatment with lysozyme confirms damage to the cell's outer membrane. Similarities between the ionic radii and charge/radius ratio cause Pb(II) to replace Ca(II) at the binding sites of lipopolysacharides, leading to rupture of protecting areas on the cell's surface. Consequently, the protection and functionality of outer membrane is lost, thus becoming the basis for the biological effect of Pb2+ on E. coli.

引用

页码：195 / 202

页数：8

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