Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

被引:81
作者
Jehlicka, Jan [1 ]
Edwards, Howell G. M. [2 ]
Osterrothova, Katerina [1 ]
Novotna, Julie [1 ]
Nedbalova, Linda [3 ]
Kopecky, Jiri [4 ]
Nemec, Ivan [5 ]
Oren, Aharon [6 ]
机构
[1] Charles Univ Prague, Inst Geochem Mineral & Mineral Resources, Prague 12843, Czech Republic
[2] Univ Bradford, Ctr Astrobiol & Extremophiles Res, Bradford BD7 1DP, W Yorkshire, England
[3] Charles Univ Prague, Dept Ecol, CR-12844 Prague, Czech Republic
[4] Acad Sci Czech Republic, Inst Microbiol, Trebon, Czech Republic
[5] Charles Univ Prague, Dept Inorgan Chem, Prague 12843, Czech Republic
[6] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Plant & Environm Sci, IL-91904 Jerusalem, Israel
来源
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES | 2014年 / 372卷 / 2030期
基金
以色列科学基金会;
关键词
detecting carotenoids; bacteria; cyanobacteria; algae; Raman spectroscopy; high-performance liquid chromatography; ATACAMA DESERT; IN-VIVO; HALOPHILIC ARCHAEA; DEAD-SEA; SP-NOV; BACTERIA; PIGMENTS; MARS; IDENTIFICATION; RELEVANCE;
D O I
10.1098/rsta.2014.0199
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.
引用
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页数:17
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