Diverse effects of G-protein-coupled free fatty acid receptors on the regulation of cellular functions in lung cancer cells

被引：27
作者：
Kita, Tsubasa [1 ]
Kadochi, Yui [1 ]
Takahashi, Kaede [1 ]
Fukushima, Kaori [1 ]
Yamasaki, Eri [1 ]
Uemoto, Taiki [1 ]
Hirane, Miku [1 ]
Fukushima, Nobuyuki [2 ]
Honoki, Kanya [3 ]
Tsujiuchi, Toshifumi [1 ]
机构：
[1] Kinki Univ, Fac Sci & Engn, Dept Life Sci, Div Mol Oncol, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan
[2] Kinki Univ, Fac Sci & Engn, Dept Life Sci, Div Mol Neurobiol, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan
[3] Nara Med Univ, Dept Orthoped Surg, 840 Shijo Cho, Kashihara, Nara 6348521, Japan
来源：
EXPERIMENTAL CELL RESEARCH | 2016年 / 342卷 / 02期
关键词：
GPR120; GPR40; Cell motility; Invasion; Lung cancer; PANCREATIC BETA-CELLS; INSULIN-SECRETION; GPR40; GPR120; IDENTIFICATION; EXPRESSION;
D O I：
10.1016/j.yexcr.2016.03.008
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Free fatty acids (FFAs) are dietary nutrients which mediate a variety of biological effects through binding to G-protein-coupled FFA receptors (FFARs). G-protein-coupled receptor 120 (GPR120) and GPR40 are identified as FFARs for long- and medium-chain fatty acids. Here we investigated whether GPR120 and GPR40 are involved in the acquisition of malignant properties in lung cancer cells. Three lung cancer RLCNR, LL/2 and A549 cells used in this study expressed GPR120 and GPR40 genes. The cell motile activities of all cells were significantly suppressed by a GPR40 antagonist GW1100. In addition, GPR40 knockdown inhibited the cell motile activity of A549 cells. In gelatin zymography, matrix metalloproteinase-2 (MMP-2) activity in GPR40 knockdown was significantly lower than that in control cells. Next, to evaluate effects of GPR120 and GPR40 on cellular functions induced by anti-cancer drug, the long-term cisplatin (CDDP) treated (A549-CDDP) cells were generated. The expression levels of GPR120 and GPR40 were significantly decreased in A549-CDDP cells. While A549-CDDP cells showed the high cell motile activity, GW1100 suppressed the cell motile activity of A549-CDDP cells. These results demonstrate that GPR120 negatively and GPR40 positively regulate cellular functions during tumor progression in lung cancer cells. (C) 2016 Elsevier Inc. All rights reserved.
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页码：193 / 199
页数：7
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