Enzyme-amplified lanthanide luminescence assay of alkaline phosphatase.

被引：0
|
作者：
Zhao, QR
Li, MJ
Liu, J
Song, NL
Zhuang, XL
Chen, A
Lin, H
机构：
[1] Chinese Acad Med Sci, Inst Radiat Med, Tianjin 300192, Peoples R China
[2] Peking Union Med Coll, Tianjin 300192, Peoples R China
来源：
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1998年 / 25卷 / 01期
关键词：
enzyme-amplified lanthanide luminescence assay; enzyme-amplified time-resolved fluorescence assay; alkaline phosphatase;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme-amplified lanthanide luminescence assay of alkaline phosphatase was described. Optimization of important factors in this assay was studied. Improvement of stability of signal/noise ratio by using methyl silicon oil (I) was first reported. The sensitivity and the accuracy of the assay were 4 U/L and < 10%, respectively. The concentration range corresponding to 10% precision was 2.00 similar to 3.02 x 10(2) U/L. The activity of alkaline phosphatase in serum was also detected and the recovery was 93% similar to 95%. 5-fluorosalicyl phosphate was synthesized and used as substrate.
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页码：71 / 74
页数：4
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