Potato cultivar identification using molecular markers

被引：6
|
作者：
Rosa, Patricia Marchezi [1 ]
de Campos, Tatiana [1 ]
Barbosa de Sousa, Adna Cristina [1 ]
Sforca, Danilo Augusto [1 ]
Montan Torres, Gisele Abigail [2 ]
de Souza, Anete Pereira [3 ]
机构：
[1] Univ Estadual Campinas, UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
[2] Embrapa Trigo, BR-99001970 Passo Fundo, Rio Grande Sul, Brazil
[3] Univ Estadual Campinas, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazil
来源：
PESQUISA AGROPECUARIA BRASILEIRA | 2010年 / 45卷 / 01期
关键词：
Solanum; AFLP; genetic certification; varietal protection; variety identification; RAPD; SOLANUM-TUBEROSUM L; MICROSATELLITES; GERMPLASM; AFLP; SSR;
D O I：
10.1590/S0100-204X2010000100015
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
引用
收藏
页码：110 / 113
页数：4
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