A preliminary study on the antibacterial mechanism of Tegillarca granosa hemoglobin by derived peptides and peroxidase activity

被引：36
作者：
Bao, Yongbo [1 ]
Wang, Juanjuan [1 ,2 ]
Li, Chenghua [2 ]
Li, Peifen [1 ,2 ]
Wang, Sufang [1 ]
Lin, Zhihua [1 ]
机构：
[1] Zhejiang Wanli Univ, Zhejiang Key Lab Aquat Germplasm Resources, Coll Biol & Environm Sci, Ningbo 315100, Zhejiang, Peoples R China
[2] Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2016年 / 51卷
关键词：
Tegillarca granosa; Hemoglobin; Peptides; MIC; Antibacterial activity; Peroxidase activity; TICK BOOPHILUS-MICROPLUS; CELL-FREE HEMOGLOBIN; AMINO-ACID-SEQUENCE; ANTIMICROBIAL PEPTIDES; BOVINE HEMOGLOBIN; SCAPHARCA-INAEQUIVALVIS; ANTIOXIDANT PROPERTIES; LUCINA-PECTINATA; PHENOL OXIDASE; PROTEIN;
D O I：
10.1016/j.fsi.2016.02.004
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The blood clam, Tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (Hb). In the present study, we purified two types of T. granosa hemoglobin, Tg-HbI and Tg-HbII, using size exclusion chromatography and measured their antibacterial and peroxidase activities. We also tested antibacterial activities of peptides prepared by trypsin digestion of purified Tg-Hb and reversed -phase high-performance liquid chromatography purification. Purified Tg-HbI and Tg-HbII showed antibacterial activity against Escherichia coli, Pseudomonas putida, Bacillus subtilis, and Bacillus firmus, with differences in minimal inhibitory concentrations (MICs), but lacked antibacterial activity against Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio harveyi and Staphylococcus aureus. In contrast, 7 Tg-Hb derived peptides exhibited varying degrees of antibacterial activity against V. alginolyticus (MICs: 12-200 mu g/ml), V parahaemolyticus (11-100 mu g/ml) and V harveyi (1-200 mu g/ml). The antibacterial activity of Hb derived peptides was confirmed by fluorescence microscopy. In addition, peroxidase activity was detected in Tg-HbI and Tg-HbII. The results indicated that in addition to functioning as a respiratory protein T. granosa hemoglobins likely play a role in host antibacterial defense probably via a peroxidase activity of native molecules and some internal peptides released from the proteins. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：9 / 16
页数：8
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