Application of confocal laser scanning microscopy to the study of amber bioinclusions

被引：79

作者：

Fu, Yan-Zhe ^{[1
,2
]}

Li, Yan-Da ^{[1
]}

Su, Yi-Tong ^{[1
]}

Cai, Chen-Yang ^{[1
]}

Huang, Di-Ying ^{[1
]}

机构：

[1] Chinese Acad Sci, Ctr Excellence Lift & Paleoenvironm, Nanjing Inst Geol & Palaeontol, State Key Lab Palaeobiol & Stratig, Nanjing 210008, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

PALAEOENTOMOLOGY | 2021年 / 4卷 / 03期

基金：

中国国家自然科学基金;

关键词：

arthropod inclusions; CLSM imaging system; fluorescence; fossil; micro-CT;

D O I：

10.11646/palaeoentomology.4.3.14

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Confocal laser scanning microscopy is an essential analytical tool in biological, biomedical, and material sciences, integrating microscope manufacturing technology, optical-electronic technology, and computer technology. In the last decade, confocal laser scanning microscopy has been successfully applied to the study of amber bioinclusions. Enhanced signal to noise ratios, resolution power, capability of optical sectioning, three-dimensional reconstruction, and better performance when imaging thicker samples provide a great deal of valuable and detailed morphological information about amber fossils. We briefly discuss the practical applications of CLSM in amber studies and compare it with other imaging methods commonly used in the field, including bright-field microscopy, wide-field fluorescence microscopy, and micro-computed tomography. A general procedure for imaging amber inclusions with CLSM is provided, with a focus on pretreatments and image processing.

引用

页码：266 / 278

页数：13

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