Complete mitochondrial genome and phylogenetic analysis of Anabarilius grahami (Teleostei, Cyprinidae, Cultrinae)

The complete mitochondrial DNA genome of Anabarilius grahami was determined by the primer walking sequence method. The entire mitochondrial genome is 16,612 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and one control region (CR). As in other teleosts, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall nucleotide composition was 28.9% A, 24.8% T, 27.9% C, and 18.3% G, respectively, with a slight A+T bias (53.7%). Phylogenetic analysis based on 12 concatenated protein-coding genes of the heavy strand shows the evolutionary position of A. grahami is more closely related to two species of the genus Hemiculter within the subfamily Cultrinae.