Halo-tag mediated self-labeling of fluorescent proteins to molecular beacons for nucleic acid detection

被引：23

作者：

Blackstock, Daniel ^{[1
]}

Chen, Wilfred ^{[1
]}

机构：

[1] Univ Delaware, Dept Biomol & Chem Engn, Newark, DE 19716 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 89期

基金：

美国国家科学基金会;

关键词：

LIVING CELLS; BINDING PROTEINS; FRET; DNA; DYNAMICS; CONJUGATION; DELIVERY; GFP;

D O I：

10.1039/c4cc07118b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report here the generation of a fluorescent protein (FP)-based dual molecular beacon (MB) system for nucleic acid detection. Halo-tag mediated conjugation was used for the site-specific decoration of MBs with two different FP fusions, thereby enabling easy detection of target sequences by fluorescence resonance energy transfer or FRET. Enhanced intracellular delivery was demonstrated by simply tethering a well-known TAT peptide sequence to the N-terminus of the fusion proteins.

引用

页码：13735 / 13738

页数：4

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