A chemical-genetic approach for precise spatio-temporal control of cellular signaling

被引：43

作者：

Dong, Shuyun ^{[1
]}

Allen, John A. ^{[1
]}

Farrell, Martilias ^{[1
]}

Roth, Bryan L. ^{[1
]}

机构：

[1] Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC 27599 USA

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 08期

关键词：

PROTEIN-COUPLED RECEPTORS; DESIGNED G(I)-COUPLED RECEPTOR; 5-HT2A SEROTONIN RECEPTORS; CONDITIONAL EXPRESSION; TRANSGENIC MICE; IN-VIVO; NEURONS; TRANSDUCTION; ACTIVATION; CHANNELS;

D O I：

10.1039/c002568m

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently we have perfected a chemical-genetic approach to gain precise spatio-temporal control of cellular signaling. This approach entails the cell-type specific expression of mutant G-protein coupled receptors which have been evolved to be activated by the pharmacologically inert drug-like small molecule clozapine N-oxide. We have named these mutant GPCRs DREADDs (Designer Receptors Exclusively Activated by Designer Drugs). In this paper we will first review recent applications of this technology for the remote control of neuronal and non-neuronal signaling. Next, we will also introduce new variants which could be useful for the control of cellular signaling in discrete cellular compartments. Finally, we will suggest future basic science and therapeutic applications of this general technology.

引用

页码：1376 / 1380

页数：5

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