Muscle contraction accelerates IL-6 mRNA expression in the rat masseter muscle

被引:14
作者
Ono, Tsuyoshi
Maekawa, Kenji
Watanabe, Shogo
Oka, Hisao
Kuboki, Takuo
机构
[1] Okayama Univ, Grad Sch Med Dent & Pharmceut Sci, Dept Oral & Maxillofacial Rehabil, Okayama 7008525, Japan
[2] Okayama Univ, Grad Sch Hlth Sci, Okayama 7008525, Japan
[3] Okayama Univ, Sch Med, Fac Hlth Sci, Okayama 7008525, Japan
关键词
masseter muscle; interleukin-6 (IL-6); muscle contraction;
D O I
10.1016/j.archoralbio.2006.10.025
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objective: : This study was conducted to determine if interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) mRNA expression increase in response to muscle contraction caused by repetitive electrical stimulation of the rat masseter muscle. Methods: : Male Wistar rats weighing 140-160 g were divided randomly into the following three groups: electrical stimulation (ES) group (n = 21), carrageenan injection (CI) group (n = 24), and ES under dantrolene sodium (muscle relaxant) injection (ESDI) group (n = 7). ES or CI was done to the left masseter; and mock ES or mock Cl to the right. Muscle tissues on both sides were sampled for total RNA isolation. Real-time RT-PCR was performed, with the cyclophilin A (CypA) mRNA level in each sample as an internal control. Mean relative IL-6 (il-6/cypA) and IL-1 beta (il-1 beta/cypA) mRNA levels were compared between the experimental and mock-treated sides within each group. Results: Mean IL-6/CypA levels in the ES- or Cl-treated muscle significantly increased, without any significant incremental change observed in either mock-treated muscle. Interestingly, the increase in the il-6/cypA level caused by the ES was suppressed by the injection of dantrolene sodium in the ESDI group. Furthermore, the mean il-1 beta/cypA level in the Cl-treated masseter also significantly increased without any significant incremental change observed in the mock-treated muscle. However, there was no significant difference in the mean il-1 beta/cypA levels in the masseter between the ES- and the mock-treated sides. Conclusions: : These results show that IL-6 mRNA expression in the rat masseter muscle was accelerated by the CI or by repetitive muscle contraction induced by ES. Since the mRNA level of IL-1 beta, a well-known proinflammatory cytokine, was not altered by the contraction, the accelerated IL-6 mRNA expression elicited by the muscle contraction does not seem to be related to local inflammation. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:479 / 486
页数:8
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