Cytotoxic effects of two antimolting insecticides in mammalian CHO-K1 cells

被引：19
作者：
Bayoumi, AE
Pérez-Pertejo, Y
Zidan, HZ
Balaña-Fouce, R
Ordóñez, C
Ordóñez, D [1 ]
机构：
[1] Univ Leon, Dept Pharmacol & Toxicol, INTOXCAL, Fac Med Vet, Leon 24011, Spain
[2] Ain Shams Univ, Fac Agr, Dept Plant Protect, Cairo, Egypt
来源：
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2003年 / 55卷 / 01期
关键词：
integrated pest management; antimolting agents; cytotoxicity; diflubenzuron; pyriproxyfen;
D O I：
10.1016/S0147-6513(02)00068-4
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Cytotoxicity of two insect growth regulators, diflubenzuron, a benzoylphenylurea derivative that inhibits the synthesis of new chitin in target organisms, and pyriproxyfen, an insect juvenile hormone analogue, were tested on CHO-K1 cultures, using the neutral red incorporation assay. Both compounds displayed cytotoxic effects that rise with time exposure. The presence of either fetal calf serum or bovine serum albumin diminished significantly the cytotoxicity of both compounds, thus pointing to a strong protein binding. In addition, extensive metabolization with rat liver submitochondrial fraction gave rise to metabolites less toxic than the parent compounds, implying the relative safety of both diflubenzuron and pyriproxyfen in mammals. (C) 2003 Elsevier Science (USA). All rights reserved.
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页码：19 / 23
页数：5
相关论文
共 26 条
[1]  
[Anonymous], TOXICOLOGICAL EVALUA, DOI DOI 10.1007/b136016
[2]   Basal cytotoxicity of four insect sex pheromones in CHO-K1 cells [J].
Bayoumi, AE ;
Ordónez, C ;
Pérez-Pertejo, Y ;
Zidan, HZ ;
Balaña-Fouce, R ;
Reguera, RM ;
Escudero, DO .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2002, 68 (02) :302-308
[3]   TOXICITY DETERMINED INVITRO BY MORPHOLOGICAL ALTERATIONS AND NEUTRAL RED ABSORPTION [J].
BORENFREUND, E ;
PUERNER, JA .
TOXICOLOGY LETTERS, 1985, 24 (2-3) :119-124
[4]   Advances in plant health management in the twentieth century [J].
Cook, RJ .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2000, 38 :95-116
[5]   Cytotoxic effects and induction of cytochromes P450 1A1/2 by insecticides, in hepatic or epidermal cells: binding capability to the Ah receptor [J].
Delescluse, C ;
Ledirac, N ;
de Sousa, G ;
Pralavorio, M ;
Lesca, P ;
Rahmani, R .
TOXICOLOGY LETTERS, 1998, 96-7 :33-39
[6]   INHIBITION OF CHITIN SYNTHESIS BY 2 1-(2,6-DISUBSTITUTED BENZOYL)-3-PHENYLUREA INSECTICIDES .2. [J].
DEUL, DH ;
JONG, BJD ;
KORTENBACH, JAM .
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 1978, 8 (01) :98-105
[7]   New insecticides with ecdysteroidal and juvenile hormone activity [J].
Dhadialla, TS ;
Carlson, GR ;
Le, DP .
ANNUAL REVIEW OF ENTOMOLOGY, 1998, 43 :545-569
[8]  
Finney D.J., 1952, Statistical method in biological assay
[9]  
HOFS HP, 1991, INVEST NEW DRUG, V9, P227
[10]  
JENKINS VK, 1986, INVEST NEW DRUG, V4, P325
123