Identification and characterization of Serratia marcescens ZJB-09104, a nitrile-converting bacterium

被引：15

作者：

Jin, Li-Qun ^{[1
]}

Liu, Zhi-Qiang ^{[1
]}

Zheng, Yu-Guo ^{[1
]}

Shen, Yin-Chu ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310032, Zhejiang, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2010年 / 26卷 / 05期

关键词：

Serratia marcescens; Nitrile hydratase; 2-Cyanopyrazine; Pyrazinamide; EPOXIDE HYDROLASE; HYDRATASE; PYRAZINAMIDE; EXPRESSION; EVOLUTION; CLONING;

D O I：

10.1007/s11274-009-0238-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pyrazinamide has received considerable attention for its effective antibacterial action in the reappearance of tuberculosis and for its broad application in the chemical industry. In this study, a 2-cyanopyrazine-degrading bacterial strain, numbered ZJB-09104, was newly isolated and identified as Serratia marcescens, based on its physiological and biological tests, ATB system analysis, and 16S rDNA sequence analysis. The strain exhibits only nitrile hydratase (NHase) activity and this NHase belongs to the cobalt NHase family of enzymes. Thermostability tests suggested that the NHase is thermophilic with an optimum temperature of 50A degrees C. The NHase was effective in converting nitriles to the corresponding amides under the conditions of temperature 50A degrees C and time course 7 h, respectively.

引用

页码：817 / 823

页数：7

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