Genome-wide comparative analysis ofbone morphogenetic proteins: genomic structure, phylogeny, and expression patterns in the golden pompano,Trachinotus ovatus(Linnaeus, 1758)

被引:7
作者
Sun, Jinhui [1 ]
Zhu, Kecheng [2 ,3 ,4 ]
Guo, Huayang [2 ,3 ,4 ]
Zhang, Nan [2 ,3 ,4 ]
Jiang, Shigui [2 ,3 ,4 ]
Zhang, Dianchang [2 ,3 ,4 ]
机构
[1] Tianjin Agr Univ, Coll Fisheries, Tianjin, Peoples R China
[2] Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China
[3] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
[4] Guangdong Prov Engineer Technol Res Ctr Marine Bi, 231 Xingang Rd West, Guangzhou 510300, Guangdong, Peoples R China
关键词
Trachinotus ovatus; Bone morphogenetic protein; Phylogenetic analysis; Gene expression; MOLECULAR CHARACTERIZATION; GENE-EXPRESSION; SPATIOTEMPORAL EXPRESSION; BETA SUPERFAMILY; COMMON CARP; BONE; GROWTH; BMP15; DIFFERENTIATION; FIN;
D O I
10.1007/s10695-019-00721-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bone morphogenetic proteins (BMPs) play important roles in various physiological processes, especially during the formation and maintenance of various organs. In this study, we first obtained and characterized twenty BMP genes from the Trachinotus ovatus genome (designated as ToBMPs). Sequence alignment and phylogenetic analysis both indicated that the predicted amino acid sequences of ToBMP were highly conserved with corresponding homologs of other species. Moreover, a comparative analysis was performed with seven representative vertebrate genomes and found difference in number of BMP3 genes in different species, which three members, BMP3a, BMP3b-1, and BMP3b-2, existed in diploid T. ovatus, but there were four and two members in tetraploidized Cyprinus carpio (BMP3a-1, BMP3a-2, BMP3b-1, and BMP3b-2) and diploid Danio rerio (BMP3a and BMP3b), respectively. The amino acid alignment and genomic structure analysis of ToBMP3 also suggested that the BMP3 gene had expanded in T. ovatus. Furthermore, tissue expression patterns were assessed for the small intestine, liver, white muscle, brain, spleen, fin, gill, head kidney, stomach, blood, and gonads. It was discovered that BMP1, BMP2, BMP3a, BMP4, BMP6, BMP7b, BMP11, and BMP16 were ubiquitously expressed in all the tissues tested. To study the regulatory function of BMP in response to the intake of different types of food, the expression changes in BMP mRNAs were detected by qRT-PCR, and the results showed that the majority of the BMP genes had the highest mRNA levels in the small intestine and liver after ingesting pelleted feed. Our data provide a useful resource for further studies on how paralogous genes may have different expression profiles in T. ovatus.
引用
收藏
页码:345 / 358
页数:14
相关论文
共 57 条
[1]   Attenuated BMP1 Function Compromises Osteogenesis, Leading to Bone Fragility in Humans and Zebrafish [J].
Asharani, P. V. ;
Keupp, Katharina ;
Semler, Oliver ;
Wang, Wenshen ;
Li, Yun ;
Thiele, Holger ;
Yigit, Goekhan ;
Pohl, Esther ;
Becker, Jutta ;
Frommolt, Peter ;
Sonntag, Carmen ;
Altmueller, Janine ;
Zimmermann, Katharina ;
Greenspan, Daniel S. ;
Akarsu, Nurten A. ;
Netzer, Christian ;
Schoenau, Eckhard ;
Wirth, Radu ;
Hammerschmidt, Matthias ;
Nuernberg, Peter ;
Wollnik, Bernd ;
Carney, Thomas J. .
AMERICAN JOURNAL OF HUMAN GENETICS, 2012, 90 (04) :661-674
[2]  
Bahamonde ME, 2001, J BONE JOINT SURG AM, V83A, pS56
[3]   BMP signalling: agony and antagony in the family [J].
Brazil, Derek P. ;
Church, Rachel H. ;
Surae, Satnam ;
Godson, Catherine ;
Martin, Finian .
TRENDS IN CELL BIOLOGY, 2015, 25 (05) :249-264
[4]   BMP-9 interferes with liver regeneration and promotes liver fibrosis [J].
Breitkopf-Heinlein, Katja ;
Meyer, Christoph ;
Koenig, Courtney ;
Gaitantzi, Haristi ;
Addante, Annalisa ;
Thomas, Maria ;
Wiercinska, Eliza ;
Cai, Chen ;
Li, Qi ;
Wan, Fengqi ;
Hellerbrand, Claus ;
Valous, Nektarios A. ;
Hahnel, Maximilian J. ;
Ehlting, Christian ;
Bode, Johannes G. ;
Mueller-Bohl, Stephanie ;
Klingmueller, Ursula ;
Altenoeder, Jutta ;
Ilkavets, Iryna ;
Goumans, Marie-Jose ;
Hawinkels, Lukas J. A. C. ;
Lee, Se-Jin ;
Wieland, Matthias ;
Mogler, Carolin ;
Ebert, Matthias P. ;
Herrera, Blanca ;
Augustin, Hellmut G. ;
Sanchez, Aranzazu ;
Dooley, Steven ;
ten Dijke, Peter .
GUT, 2017, 66 (05) :939-954
[5]   The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[6]   Pleiotropic effects of the bone morphogenetic proteins on development of the enteric nervous system [J].
Chalazonitis, Alcmene ;
Kessler, John A. .
DEVELOPMENTAL NEUROBIOLOGY, 2012, 72 (06) :843-856
[7]   BMP10 is essential for maintaining cardiac growth during murine cardiogenesis [J].
Chen, HY ;
Shi, S ;
Acosta, L ;
Li, WM ;
Lu, J ;
Bao, SD ;
Chen, ZA ;
Yang, ZC ;
Schneider, MD ;
Chien, KR ;
Conway, SJ ;
Yoder, MC ;
Haneline, LS ;
Franco, D ;
Shou, WN .
DEVELOPMENT, 2004, 131 (09) :2219-2231
[8]   Genome wide identification, phylogeny, and expression of bone morphogenetic protein genes in tetraploidized common carp (Cyprinus carpio) [J].
Chen, Lin ;
Dong, Chuanju ;
Kong, Shengnan ;
Zhang, Jiangfan ;
Li, Xuejun ;
Xu, Peng .
GENE, 2017, 627 :157-163
[9]   Human BMP-7/OP-1 induces the growth and differentiation of adipocytes and osteoblasts in bone marrow stromal cell cultures [J].
Chen, TL ;
Shen, WJ ;
Kraemer, FB .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2001, 82 (02) :187-199
[10]   Expression of bmp2a and bmp2b in late-stage zebrafish median fin development [J].
Crotwell, PL ;
Sommervold, AR ;
Mabee, PM .
GENE EXPRESSION PATTERNS, 2004, 5 (02) :291-296