Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

被引：15

作者：

Tzeng, Jam-Inn ^{[2
,3
]}

Chen, Bing-Chang ^{[4
]}

Chang, Huey-Mei ^{[1
]}

Wang, Jhi-Joung ^{[2
]}

Sureshbabu, Munisamy ^{[1
]}

Chien, Ming-Hsien ^{[1
]}

Hsu, Ming-Jen ^{[1
,5
]}

Bien, Mauo-Ying ^{[4
,6
]}

Chiu, Wen-Ta ^{[7
]}

Hong, Chuang-Ye ^{[8
]}

Lin, Chien-Huang ^{[1
,7
,8
]}

机构：

[1] Taipei Med Univ, Coll Med, Grad Inst Med Sci, Taipei 110, Taiwan

[2] Chi Mei Med Ctr, Dept Anesthesiol, Tainan, Taiwan

[3] Taipei Med Univ, Coll Med, Dept Anesthesiol, Taipei 110, Taiwan

[4] Taipei Med Univ, Coll Med, Sch Resp Therapy, Taipei 110, Taiwan

[5] Taipei Med Univ, Coll Med, Dept Pharmacol, Taipei 110, Taiwan

[6] Taipei Med Univ Hosp, Dept Resp Therapy, Taipei, Taiwan

[7] Taipei Med Univ, Shuang Ho Hosp, Jhonghe, Taipei County, Taiwan

[8] Taipei Med Univ, Wang Fang Hosp, Taipei 110, Taiwan

来源：

PHARMACOLOGICAL RESEARCH | 2010年 / 61卷 / 02期

关键词：

COX-2; Inflammation; NF-kappa B; Protein kinase C; Peptidoglycan; PULMONARY EPITHELIAL-CELLS; PHOSPHATIDYLINOSITOL; 3-KINASE; BACTERIAL PEPTIDOGLYCAN; LIPOTEICHOIC ACID; P38; MAPK; TRANSCRIPTION; INDUCTION; SYNTHASE; SIGNAL; ZETA;

D O I：

10.1016/j.phrs.2009.09.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-kappa B (NF-kappa B) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in kappa B-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-kappa B activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：162 / 166

页数：5

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