Inhibitory effects of various plant polyphenols on the toxicity of Staphylococcal α-toxin

被引：41
作者：
Choi, Oksun
Yahiro, Kinnosuke
Morinaga, Naoko
Miyazaki, Masaru
Noda, Masatoshi
机构：
[1] Chiba Univ, Grad Sch Med, Dept Mol Infectiol, Chiba 2608670, Japan
[2] Chiba Univ, Grad Sch Med, Dept Gen Surg, Chiba 2608670, Japan
来源：
MICROBIAL PATHOGENESIS | 2007年 / 42卷 / 5-6期
关键词：
Staphylococcus aureus; alpha-toxin; polyphenol; hop bract tannin; apple condensed tannin;
D O I：
10.1016/j.micpath.2007.01.007
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Staphylococcal alpha-toxin, known for its wide spectrum of biological activities, is involved in the pathogenesis of Staphylococcal infectious diseases. In recent years, various phytochemicals have been found to have antimicrobiological, including antibacterial, antiviral and antifungal, and antitoxic activities. We investigated whether several plant polyphenols inhibit alpha-toxin activities in vitro and in vivo. We found hop bract tannin (HBT) and apple condensed tannin (ACT) to exert inhibitory effects on alpha-toxin cytotoxicity. HBT also reduced the murine skin inflammatory effect and the lethality of alpha-toxin. These polyphenols formed aggregates with alpha-toxin and thereby inhibited its activities. Inhibition of alpha-toxin by HBT and ACT was dose dependent, suggesting that these polyphenols may be a useful adjunct to current treatments for alpha-toxin catalyzed Staphylococcal infectious diseases. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：215 / 224
页数：10
相关论文
共 34 条
[1]  
Ahmad N, 2001, CLIN CANCER RES, V7, P1466
[2]   α-toxin is a mediator of Staphylococcus aureus-induced cell death and activates caspases via the intrinsic death pathway independently of death receptor signaling [J].
Bantel, H ;
Sinha, B ;
Domschke, W ;
Peters, G ;
Schulze-Osthoff, K ;
Jänicke, RU .
JOURNAL OF CELL BIOLOGY, 2001, 155 (04) :637-647
[3]  
BHAKDI S, 1984, INFECT IMMUN, V46, P318, DOI 10.1128/IAI.46.2.318-323.1984
[4]   Alpha-toxin is produced by skin colonizing Staphylococcus aureus and induces a T helper type 1 response in atopic dermatitis [J].
Breuer, K ;
Wittmann, M ;
Kempe, K ;
Kapp, A ;
Mai, U ;
Dittrich-Breiholz, O ;
Kracht, M ;
Mrabet-Dahbi, S ;
Werfel, T .
CLINICAL AND EXPERIMENTAL ALLERGY, 2005, 35 (08) :1088-1095
[5]   Polyphenol/peptide binding and precipitation [J].
Charlton, AJ ;
Baxter, NJ ;
Khan, ML ;
Moir, AJG ;
Haslam, E ;
Davies, AP ;
Williamson, MP .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (06) :1593-1601
[6]   Spontaneous oligomerization of a staphylococcal α-hemolysin conformationally constrained by removal of residues that form the transmembrane β-barrel [J].
Cheley, S ;
Malghani, MS ;
Song, LZ ;
Hobaugh, M ;
Gouaux, JE ;
Yang, J ;
Bayley, H .
PROTEIN ENGINEERING, 1997, 10 (12) :1433-1443
[7]  
Chen JH, 2004, AM J CLIN NUTR, V80, P742
[8]   Staphylococcus aureus α-toxin-induced cell death:: predominant necrosis despite apoptotic caspase activation [J].
Essmann, F ;
Bantel, H ;
Totzke, G ;
Engels, IH ;
Sinha, B ;
Schulze-Osthoff, K ;
Jänicke, RU .
CELL DEATH AND DIFFERENTIATION, 2003, 10 (11) :1260-1272
[9]   Staphylococcal toxins and protein a differentially induce cytotoxicity and release of tumor necrosis factor-alpha from human keratinocytes [J].
Ezepchuk, YV ;
Leung, DYM ;
Middleton, MH ;
Bina, P ;
Reiser, R ;
Norris, DA .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 107 (04) :603-609
[10]   New TNF-α releasing inhibitors as cancer preventive agents from traditional herbal medicine and combination cancer prevention study with EGCG and sulindac or tamoxifen [J].
Fujiki, H ;
Suganuma, M ;
Kurusu, M ;
Okabe, S ;
Imayoshi, Y ;
Taniguchi, S ;
Yoshida, T .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 523 :119-125
1234