Serum metabolomics in mice after paraquat posioning

被引：3

作者：

Gao, Lina ^{[1
]}

Wang, Guang ^{[2
]}

Yuan, Huiya ^{[1
]}

Xu, Enyu ^{[1
]}

Liu, Guojie ^{[3
]}

Liu, Junting ^{[1
]}

机构：

[1] China Med Univ, Sch Forens Med, Shenyang 110014, Liaoning, Peoples R China

[2] China Med Univ, Dept Lab Anim Ctr, Shenyang 110014, Liaoning, Peoples R China

[3] China Med Univ, Sch Fundamental Sci, Shenyang 110014, Liaoning, Peoples R China

来源：

MOLECULAR & CELLULAR TOXICOLOGY | 2019年 / 15卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Paraquat; Toxicology; Metabolomics; Gas chromatography-mass spectrometry; DOPAMINERGIC CELL-DEATH; OXIDATIVE STRESS; METABOLISM; MITOCHONDRIAL; MORTALITY; ACID; RATS;

D O I：

10.1007/s13273-019-0049-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Backgrounds Paraquat is toxic to humans and there is no antidote for paraquat poisoning. Moreover, the mechanisms of paraquat toxicity have not been clearly defined. Methods In this study, we developed a serum metabolomic method using gas chromatography- mass spectrometry (GC-MS) to evaluate the effects of paraquat poisoning on mice. Pattern recognition analysis, including both principal component analysis and cross orthogonal partial least squares-discriminate analysis revealed that paraquat poisoning induced energy/redox cycle and metabolic perturbations. Results Compared with the control group, the levels of creatinine, citric acid, succinic acid, fumaric acid and glycine in the sub acute paraquat poisoning group increased (P < 0.05), while the levels of alpha-Tocopherol, 6-phosphogluconic acid and palmitoleic acid decreased (P < 0.05). Conclusion These findings provide an overview of systematic responses to paraquat exposure and metabolomic insight into the toxicological mechanism of paraquat. Our results demonstrate that the metabolomic method based on GC-MS can be a useful tool to elucidate the mechanism of PQ toxicity, and provide a new direction for its clinical diagnosis and treatment.

引用

页码：453 / 458

页数：6

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